通腑泄热法中药对肝缺血再灌注损伤兔的保护作用  

Protective effect of tongfu xiere method on hepatic ischemia reperfusion injury in rabbits

作　　者：李伟涛 翟淑萍 王永亮 苑伟 王百林 Li Weitao;Zhai Shuping;Wang Yongliang;Yuan Wei;Wang Bailin(Department of General Surgery, Guangzhou Red Cross Hospital, Medical College of Jinan University, Guangzhou 510220, China;Guizhou Medical University, Clinical Medical College, Guizhou 550004, China)

机构地区：[1]暨南大学医学院附属广州红十字会医院普通外科,广州510220 [2]贵州医科大学临床医学院,550004

出　　处：《中华实验外科杂志》2019年第8期1398-1400,共3页Chinese Journal of Experimental Surgery

摘　　要：目的检测兔血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的含量变化,观察通腑泄热法中药对兔肝缺血再灌注损伤的保护作用.方法制备兔肝缺血再灌注模型30例,随机分为3组,即中药组(n=10例)和假手术组(n=10例),对照组(n=10例),分别按照于术前、术中和术后5d取各组家兔的血清,用酶联免疫吸附试验(ELISA)法检测血清中TNF-α、IL-6的含量变化.结果术前、术中和术后5d的TNF-α的含量各组分别为:中药组(203.05±36.52)、(452.56±29.29)、(195.00±35.86) ng/L.对照组(219.18±41.03)、(462.45±62.17)、(322.77±70.19) ng/L.假手术组(203.63±21.32)、(210.77±58.83)、(205.19±60.39) ng/L.术前、术中和术后5d的IL-6的含量各组分别为:中药组(423.56±64.27)、(1 325.55±97.02)、(223.69±62.72) ng/L.对照组(428.08±110.75)、(1 221.99±101.21)、(851.07±58.84) ng/L.假手术组(411.02±57.02)、(377.28±168.33)、(382.92±81.07) ng/L.其中,TNF-α和IL-6的含量变化,术前各组之间差异均无统计学意义(TNF-α:t=-0.929、-0.044、1.064,P>0.05;IL-6:t=-0.112、0.462、0.433,P>0.05),术中中药组和对照组明显上升,差异有统计学意义(TNF-α:t =21.549、19.720,P<0.05;IL-6:t =21.506、18.761,P<0.05),术后5d恢复正常,中药组与对照组比较差异有统计学意义(f=-23.069,P<0.05).结论通腑泄热法可明显降低兔肝缺血再灌注术后血清NF-α、IL-6的数值,降低肝缺血再灌注损伤引起的炎性反应,从而保护兔肝缺血再灌注术后的肝功能.Objective To investigate the protective effect of Tongfu Xiere method on hepatic ischemia reperfusion injury in rabbits by examining the changes in the serum tumor necrosis factor (TNF)-α and interleukin (IL)-6. Methods The rabbit hepatic ischemia reperfusion model was established (n=30). The animals were randomly divided into three groups: Chinese medicine group (n=10), pseudo operation group (n=10) and control group (n=10). The blood serum of each group was collected separately before operation, during operation and 5 days after operation. The serum levels of TNF-α and IL-6 were measured by enzyme linked immunosorbent assay (ELISA). Results Before operation, during operation and 5 days after operation, the TNF-α levels were as follows: Chinese medicine group [(203.05±36.52),(452.56±29.29) and (195.00±35.86) ng/L];control group [(219.18±41.03),(462.45±62.17) and (322.77±70.19) ng/L];and pseudo operation group [(203.63±21.32),(210.77±58.83) and (205.19±60.39) ng/L], and those of IL-6 were as follows: Chinese medicine group [(423.56±64.27),(1 325.55±97.02) and (223.69±62.72) ng/L];control group [(428.08±110.75),(1 221.99±101.21) and (851.07±58.84) ng/L], and pseudo operation group [(411.02±57.02),(377.28±168.33) and (382.92±81.07) ng/L]. There was no statistically significant difference among three groups in TNF-α and IL-6 before operation (TNF-α: t=-0.929,-0.044, 1.064, P>0.05;IL-6: t=-0.112, 0.462, 0.433, P>0.05). But there was statistical significance (TNF-α: t=21.549, 19.720, P<0.05;IL-6: t=21.506, 18.761, P<0.05)with Chinese medicine group and control group during operation in which the contents of these two groups were higher. Five days after operation the figures of all groups back to normal, but the Chinese medicine group are lower than the control group with statistical significance (t=-23.069, P<0.05). Conclusion Tongfu Xiere method may significantly reduce the TNF-α and IL-6 levels in the rabbit hepatic ischemia reperfusion model and alleviate the inflammatory reaction of hepati

关 键 词：肝缺血再灌注损伤 通腑泄热法 肿瘤坏死因子-Α 白细胞介素-6 兔 

分 类 号：R285.5[医药卫生—中药学]