VEGF基因沉默对人肺癌A549细胞放射增敏作用的体内研究  

Investigation on Radiosensitivity Impact of Lung Cancer A549 Cells by Vascular Endothelial Growth Factor Silencing in vivo

作　　者：马军 罗居东 景文江 张淑莲 王娟毅 范志刚 MA Jun;LUO Jun-dong;JING Wen-jiang;ZHANG Shu-lian;WANG Juan-yi;FAN Zhi-gang(Department of Oncology,Hanzhong 3201 Hospital,Hanzhong 723000,China;Tumor Center,Changzhou Second People's Hospital Affiliated to Nanjing Medical University,Changzhou 213164,China)

机构地区：[1]汉中三二O-医院肿瘤科,汉中723000 [2]南京医科大学附属常州第二人民医院肿瘤中心,常州213164

出　　处：《实验动物与比较医学》2019年第4期280-286,共7页Laboratory Animal and Comparative Medicine

基　　金：江苏省科技厅重点研发项目(BE2018643)

摘　　要：目的探讨RNA干扰血管内皮生长因子(VEGF)基因表达载体siVEGF对人肺腺癌A549细胞荷瘤鼠的放射敏感性的影响。方法用体外培养人肺腺癌A549细胞株建立荷瘤裸小鼠模型,荷瘤鼠瘤内注射siVEGF/RNAi-MateTM脂质体混合物,再经放射治疗24 d后,处死裸小鼠,剥离瘤体称重,利用实时定量PCR (q-PCR)、免疫组织化学和Western blotting检测VEGF的基因和蛋白表达水平,评价siVEGF对放射敏感性的影响。结果 q-PCR、Western blotting和免疫组织化学检测结果表明siVEGF可显著降低VEGF的蛋白水平(P<0.05)。与对照组相比si VEGF联合放疗组的肿瘤生长和坏死程度存在显著性差异(P<0.05)。结论 siVEGF合并放射治疗能显著抑制A549细胞移植瘤的生长,增加其对放射治疗的敏感性。Objective To research the radiosensitivity of A549 cells in tumor-bearing mouse model by knock down vascular endothelial growth factor(VEGF) gene. Methods The tumorbearing mouse model was established by using A549 cells which was cultured in vitro. The siVEGF/RNAi-MateTM mixture was injected into the tumor of model mice and with radiotherapy. The nude mice were euthanasia on day 24 after radiotherapy. The expressions of VEGF were detected by immuno-histochemical, Q-PCR and Western blotting. Results Both the protein and mRNA levels of VEGF in VEGF knockdown and radiotherapy group were significantly decreased compared with the control group(P<0.05). The volume of tumor was decreased and the necrotic area of tumor were increased distinctly in siVEGF transfected and radiotherapy group compared with control group(P<0.05). Conclusion Knock down of VEGF and radiotherapy can inhibit tumor growth, and increase the radiosensitivity of lung cancer radiotherapy.

关 键 词：人肺腺癌 A549细胞 沉默RNA 血管内皮生长因子(VEGF) 放射敏感性 

分 类 号：Q95-33[生物学—动物学]