连续施用沼液水稻油菜轮作耕层土壤的细菌多样性PCR-DGGE分析  

Bacterial Diversity in Topsoil of Continuous Application of Biogas Slurry Rice-rape Rotation Analysised Using PCR-DGGE

作　　者：冯丹妮 伍钧[2] 杨虎德[1] FENG Dani;WU Jun;YANG Hude(Institute of Soil Fertilizer and Water-saving Agriculture,Gansu Academy of Agricultural Sciences,Lanzhou Gansu 730070,China;Sichuan Agricultural University,Chengdu Sichuan,611130,China)

机构地区：[1]甘肃省农业科学院土壤肥料与节水农业研究所,甘肃兰州730070 [2]四川农业大学,四川成都611130

出　　处：《甘肃农业科技》2019年第8期41-49,共9页Gansu Agricultural Science and Technology

摘　　要：采用PCR-DGGE克隆测序技术,分析沼液连续施用水稻油菜轮作耕层土壤细菌群落结构、丰富度指数和多样性指数等。通过DGGE图谱分析得出,沼液连年施用不利于丰富土壤细菌群落多样性,当连续3a沼液施用总量控制为339.31~396.81t/hm2时,细菌群落多样性增加,种群功能丰富、信息复杂、种群稳定,过高或过低施用沼液都会显著降低细菌群落多样性;通过UPGMA分析表明,长期连续施用沼液后土壤细菌遗传相似性降低,细菌种群结构变化越来越大。PCR-DGGE cloning sequencing technology was used to analyze and compare the bacterial community structure,richness index and diversity index of soil bacteria in rice-rape rotation cultivation layer.Through the analysis of the DGGE profiles concluded that biogas slurry in successive years application does not favor the rich soil bacterial community diversity,when biogas slurry application of total amount controled in 339.31~396.81 t/hm2,bacterial community diversity increases,bacterial population feature rich,complicated information and population stable,too high or too low application of biogas slurry can significantly reduce the bacterial community diversity;According to UPGMA analysis,the genetic similarity of soil bacteria decreased after long-term continuous application of biogas slurry,and the bacterial population structure changed bigger and bigger.

关 键 词：水稻 油菜 轮作 沼液施用量 土壤细菌多样性 PCR-DGGE 

分 类 号：S154.32[农业科学—土壤学]