金匮肾气丸联合玉屏风散补肾益气对慢阻肺大鼠IL-8、TNF-α、MMP-9、P-P65和IKB-α的影响  

Effects of Jingui Shenqi Pills with Yupingfeng San on IL-8,TNF-α,MMP-9,P-P65 and IKB-α in rats with chronic obstructive pulmonary disease

作　　者：叶玲 林小妹 YE Ling;LIN Xiao-Mei(The Affiliated People′s Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou 350003,China)

机构地区：[1]福建中医药大学附属人民医院

出　　处：《中国免疫学杂志》2019年第15期1835-1839,共5页Chinese Journal of Immunology

基　　金：福建省自然科学基金项目(2017J01212)

摘　　要：目的:探讨金匮肾气丸联合玉屏风散补肾益气对烟雾暴露所致慢性阻塞性肺疾病大鼠IL-8、TNF-α、P-P65、MMP-9和IKB-α表达的影响。方法:采用香烟烟雾暴露法建立大鼠COPD模型,实验随机分为4组:正常对照组、COPD组、COPD+补肾益气组、COPD+地塞米松组,电镜下观察大鼠肺组织的病理变化,WB检测法测定大鼠肺组织P-P65及IKB-α表达变化,ELISA检测IL-8、MMP-9和TNF-α的变化。结果:模型组气道狭窄、肺泡囊状扩张,符合COPD病理改变,补肾益气组和地塞米松组有所改善;WB检测显示模型组IKB-α表达明显降低,补肾益气组和地塞米松组表达低于模型组(P<0.05);模型组P-P65表达明显升高,补肾益气组和地塞米松组表达有所下降(P<0.05);补肾益气组与地塞米松组无统计学差异(P>0.05)。ELISA结果显示模型组IL-8、MMP-9和TNF-α明显升高(P<0.05),补肾益气组和地塞米松组大鼠表达比模型组下降(P<0.05);补肾益气组与地塞米松组无统计学差异(P>0.05)。结论:补肾益气治疗可抑制P-P65蛋白激活,升高IKB-α,减少IL-8、MMP-9、TNF-α等转录产物的生成,从而减轻气道炎症反应。Objective: To investigate the effects of Bushen Yiqi on the expression of IL-8,TNF-α,P-P65,MMP-9 and IKB-α in rats with chronic obstructive pulmonary disease induced by smoke exposure. Methods: The mouse COPD model was established by cigarette smoke exposure method.The experiment was randomly divided into 4 groups:normal control group,COPD group,COPD+Bushen Yiqi group,COPD+dexamethasone group.The pathological changes of rat lung tissue were observed under electron microscope.The expressions of P-P65 and IKB-α in rat lung tissue were determined by WB assay.The changes of IL-8,MMP-9 and TNF-α were detected by ELISA. Results: In model group,airway stenosis and alveolar cystic dilatation accorded with pathological changes of COPD,which was improved in the Bushen Yiqi group and the dexamethasone group. The WB test showed that the expression of IKB-α was significantly decreased in the model group,and the expression in the Bushen Yiqi group and the dexamethasone group was lower than that in the model group.The expression of P-P65 in the model group was significantly increased,and the expression in the Bushen Yiqi group and the dexamethasone group was decreased( P <0.05).There was no significant difference between the Bushen Yiqi group and the dexamethasone group( P >0.05).ELISA results showed that IL-8,MMP-9 and TNF-α in the model group were signific-antly increased( P <0.05),and the expressions in the rats of Bushen Yiqi group and dexamethasone group were lower than those in the model group( P <0.05).There was no significant difference in the dexamethasone group( P >0.05). Conclusion: Bushen Yiqi treatment can inhibit the activation of P-P65 protein,increase IKB-α,reduce the production of IL-8,MMP-9,TNF-α and other transcription products,thereby reducing airway inflammation.

关 键 词：IL-8 TNF-Α MMP-9 P-65 IKB-α 补肾益气法 慢性阻塞性肺疾病 

分 类 号：R256[医药卫生—中医内科学]