HIV-1感染过程中外周血CD8^+CD127^+T细胞活化耗竭研究  

Status of CD8^+CD127^+T cells and their impacts during HIV-1 infection

作　　者：刘显 董原[2] 彭丽珊[3] 杨洋[3] 王登嵘[3] 伍月榕 郁晓磊 于丽 王盈[2] 肖健[3,4] LIU Xian;DONG Yuan;PENG Li-Shan;YANG Yang;WANG Deng-Rong;WU Yue-Rong;YU Xiao-Lei;YU Li;WANG Ying;XIAO Jian(The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西中医药大学第一附属医院,南宁530001 [2]上海市疾病预防控制中心,上海200336 [3]广西中医药大学,南宁530001 [4]广西高发传染病中西医结合转化医学重点实验室,南宁530001

出　　处：《中国免疫学杂志》2019年第15期1871-1875,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(81260463);国家自然科学基金青年基金(81602905);广西中医药大学2018年广西一流学科建设项目(2018XK064);上海市卫生计委青年基金(20164Y0245);广西中医药大学第一附属医院青年基金(2017QN004);广西中医药大学引进博士科研启动基金(B170044)资助

摘　　要：目的:检测HIV-1感染者CD8^+CD127^+T细胞CD38、HLA-DR和PD-1的表达水平,探讨HIV-1感染过程中CD8^+CD127^+T细胞免疫活化和耗竭的意义。方法:采集未经抗病毒治疗的HIV-1感染者63例及健康对照者16例,采用流式细胞术检测外周血单个核细胞中CD8^+CD127^+T细胞CD38、HLA-DR和PD-1表达水平。结果:与健康对照组比较,HIV-1典型进展者和慢性感染者CD8^+T细胞CD127的表达显著下降,CD8^+CD127^+T细胞CD38、HLA-DR、PD-1的表达显著升高( P <0.05);HIV-1感染者CD4计数与CD127、CD38、HLA-DR、PD-1的表达频率负相关;HIV-1感染者CD8^+CD127^+T细胞HLA-DR的表达频率与PD-1的表达正相关。结论: HIV-1感染过程中CD8^+CD127^+T细胞高活化耗竭是引起CD8^+CD127^+T细胞减少的原因。Objective: To identify the status of CD8^+CD127^+ T cells during HIV-1 infection by detecting the expression levels of CD38, HLA-DR and PD-1 in CD8^+CD127^+ T cells. Methods: Peripheral blood mononuclear cells were isolated by density gradient centrifugation from 63 patients and 19 healthy donors.The CD8^+CD127^+T cell counts and CD38,HLA-DR,PD-1 expressions on CD8^+CD127^+T cells were analysed by flow cytometry. Results: Compared with healthy donors,HIV-1 infection resulted in a decrease of the CD127 on CD8 + T cells proportion in typical progressors and chronic persons.The expression of CD38,HAAL-DR,PD-1 on CD8^+CD127^+T cells in HIV-1 patients was significantly higher than that in healthy donors;the expression of CD127,CD38,HLA-DR,PD-1 negatively correlated with CD4^+T cell counts;PD-1 was positively correlated with HLA-DR expression on CD8^+CD127^+T cells in HIV-1 infected patients. Conclusion: Disease of CD8^+CD127^+T cells was in parallel with high level of immune activation and exhaustion.

关 键 词：人类免疫缺陷病毒1型 CD8^+CD127^+T细胞 细胞程序性死亡蛋白1 免疫活化 免疫耗竭 

分 类 号：R512.91[医药卫生—内科学]