浒苔(Ulva prolifera)核糖体基因簇单元全长序列的克隆与分析  

Cloning and Analysis of Full Length Sequence of Ulva prolifera Ribosomal Gene Cluster Unit

作　　者：沈伟杰 缪晓翔 何渊[1] 韩红宾 王宗灵 穆新武 沈颂东[1] SHEN Wei-jie;MIAO Xiao-xiang;HE Yuan;HAN Hong-bin;WANG Zong-ling;MU Xin-wu;SHEN Song-dong(School of Biology & Basic Medical Sciences, Suzhou University , Suzhou 215123, China;First Institute of Oceanography, MNR, Qingdao 266061, China;College of Environmental Science and Engineering, Ocean University of China, Qingdao 266061, China;Luoma Lake Fishery Management Committee of Jiangsu Province, Suqian 223800, China)

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123 [2]自然资源部第一海洋研究所,山东青岛266061 [3]中国海洋大学海洋环境与工程学院,山东青岛266061 [4]江苏省骆马湖渔业管理委员会,江苏宿迁223800

出　　处：《海洋科学进展》2019年第3期487-494,共8页Advances in Marine Science

基　　金：国家重点研发计划——浒苔绿潮形成机理与综合防控技术研究及应用(2016YFC1402102);国家自然科学基金——条斑紫菜核糖体基因簇的研究(41276134)

摘　　要：核糖体基因簇单元全长序列对于藻类分子鉴定与分类具有重要意义,而浒苔(Ulvaprolifera)作为形成我国黄海绿潮的主要物种,其核糖体基因簇单元全长序列的克隆还未见研究报道。本研究通过分子克隆技术成功扩增了浒苔的核糖体基因簇单元全长序列。浒苔核糖体基因簇单元全长序列为8948bp,其中18SrDNA1760bp,28SrDNA3259bp,ITS1205bp,5.8SrDNA160bp,ITS2176bp和IGS3388bp。对各部分序列的碱基组成进行分析后,我们发现ITS1,ITS2和IGS序列对胞嘧啶具有明显的偏好性,而18SrDNA和28SrDNA序列对鸟嘌呤具有明显的偏好性。浒苔核糖体基因簇单元全长序列的GC含量为54.12%,其中ITS1、ITS2和IGS序列的GC含量比保守序列的GC含量高,这表明内转录单元间隔区序列和基因间间隔区序列比保守序列变异率更高,进化速率更快。另外,IGS序列中发现大量简单的直接重复序列、串联重复序列、短对称序列和回文序列。浒苔核糖体基因簇单元全长序列可为其分类及分子鉴定提供有效的工具。The full-length sequence of ribosomal gene cluster is of great significance for the molecular identification and classification of algae. However, Ulva prolifera , a major species forming the green sea tide in the Yellow Sea, in which the complete sequence of ribosomal gene cluster unit has not been reported yet. In this study, the full-length of the ribosomal gene cluster unit of Ulva prolifera has been amplified successfully by molecular cloning technology. The full-length sequence of the Ulva prolifera gene cluster was 8 948 bp, of which 18S rDNA 1 760 bp, 28S rDNA 3 259 bp, ITS1 205 bp, 5.8S rDNA 160 bp, ITS2 176 bp and IGS 3 388 bp. After analyzing the base composition of each part of the sequence, we found that ITS1、ITS2 and IGS sequences have obvious preference for cytosine, while 18S rDNA and 28S rDNA sequences have obvious preference for guanine. The GC content of the full-length sequence of Ulva prolifera gene cluster was 54.12%. The GC content of ITS1,ITS2 and IGS sequences were higher than those of conserved sequences, indicating that the internal transcribed spacer sequences and intergenic sequences have higher variation rate than the conserved sequences, the mutation rate was higher, the evolution rate was faster. In addition, a large number of simply direct repeat sequences, tandem repeats, dyad sequences and palindrome sequence were found in the IGS sequences. In conclusion, the full-length sequence of the ribosomal gene cluster unit of Ulva prolifera can provide an effective molecular tool for the classification and molecular identification of green algae.

关 键 词：浒苔 18SrDNA 28SrDNA ITS1 ITS2 5.8SrDNA IGS 核糖体基因簇 

分 类 号：S917[农业科学—水产科学]