非人灵长类个性化麻醉方案的探讨  

Exploration of an individualized anesthesia method for non-human primates

作　　者：周智刚 段志刚 王宏 龚南南 刘杰 陈兴龙 卓艳 周引 张婷 陈永昌 ZHOU Zhigang;DUAN Zhigang;WANG Hong;GONG Nannan;LIU Jie;CHEN Xinglong;ZHUO Yan;ZHOU Yin;ZHANG Ting;CHEN Yongchang(Yunnan Key Laboratory of Primate Biomedical Research;Institute of Primate Translational Medicine,Kunming University of Science and Technology,Kunming 650500,China)

机构地区：[1]云南中科灵长类生物医学重点实验室 [2]昆明理工大学灵长类转化医学研究院,昆明650500

出　　处：《中国实验动物学报》2019年第4期473-478,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金联合重点项目(U1602224)~~

摘　　要：目的 比较氯胺酮、舒泰、速眠新Ⅱ、戊巴比妥钠等4种全身麻醉药或其组合对非人灵长类的麻醉效果,探寻能替代或者减少氯胺酮使用的个性化麻醉方案。方法 以单独使用氯胺酮麻醉的方案作为对照,另设单独使用舒泰、氯胺酮复合速眠新Ⅱ、舒泰复合速眠新Ⅱ和戊巴比妥钠复合速眠新Ⅱ等麻醉4个实验组,每组选取5只食蟹猴进行实验,记录麻醉后的心率、体温、血氧饱和度、以及麻醉诱导时间和维持时间,以比较各方案的麻醉效果。结果 与单独使用氯胺酮麻醉比较,其他四种麻醉方案在心率、体温、血氧饱和度和麻醉诱导时间上均无显著性差异,不同方案麻醉维持时间分布在30~200min之间。在非人灵长类的全身麻醉中,舒泰可以很好地替代氯胺酮;氯胺酮复合速眠新Ⅱ麻醉可取得较长的麻醉维持时间,并减少氯胺酮的使用量;舒泰与速眠新Ⅱ联用、戊巴比妥钠与速眠新Ⅱ联用的方案也可替代氯胺酮,且麻醉维持时间较长。结论 在一定的麻醉时间内,联合用药可以降低氯胺酮的使用量,不同麻醉方案灵活运用可满足不同实验对麻醉维持时间的需求。Objective To meet the individual anesthesia requirements for different surgical procedures for non-human primates that can replace or reduce the use of ketamine. To this aim, we compared the effects on body temperature, heart rate and blood oxygen saturation of ketamine, Zoletil, SumianxinⅡ, pentobarbital, and their combination anesthesia in monkeys. Methods Fifteen healthy female cynomolgus monkeys were divided into the following five groups: Zoletil alone, Zoletil + Sumianxin Ⅱ, ketamine + Sumianxin Ⅱ, pentobarbital + Sumianxin Ⅱ groups, and ketamine alone as a control group(n=5 per group). After the monkeys were anesthetized, the heart rate, body temperature, blood oxygen saturation, induction time, and maintenance time of anesthesia were recorded. Results There were no significant differences in heart rate, body temperature, blood oxygen saturation, or induction time between the control group and experimental groups. The maintenance time of each group ranged from about 30 to 200 minutes. Moreover, in general anesthesia of non-human primates, ketamine can be replaced by Zoletil;Ketamine combined with Sumianxin Ⅱ can achieve a longer maintenance time and a reduced dose of ketamine;Zoletil combined with Sumianxin Ⅱ and pentobarbital combined with Sumianxin Ⅱ can also replace ketamine, and the maintenance time of these combinations is longer than ketamine. Conclusions During certain operation duration, the combined administration can reduce the dosage of ketamine, and the flexible use of different anesthesia method can meet the individualized needs of different maintenance times in general anesthesia in non-human primates.

关 键 词：全身麻醉 非人灵长类 氯胺酮 舒泰 速眠新Ⅱ 戊巴比妥钠 

分 类 号：Q95-33[生物学—动物学]