血管紧张素转换酶2在仔猪空肠中的分布表达及其与物质转运的关系  

Distribution and Expression of Angiotensin-Converting Enzyme 2 (ACE2) in the Jejunum of Piglets and Its Relationship with Substance Transport

作　　者：李志强 闫书平 纪晓霞 王凯[1] 张源淑[1] LI Zhiqiang;YAN Shuping;JI Xiaoxia;WANG Kai;ZHANG Yuanshu(Key Laboratory of Animal Physiology and Biochemistry of Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室

出　　处：《畜牧兽医学报》2019年第8期1676-1684,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30871838);江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：通过研究血管紧张素转换酶2(ACE2)与中性氨基酸转运载体B^0AT1和葡萄糖转运载体SGLT-1、GLUT-2在不同日龄仔猪肠道中的分布表达,探讨其相互关系。以断奶仔猪为研究对象,分别取21、35日龄仔猪空肠中段组织,采用免疫组化、Western blot和RT-qPCR、HPLC等方法,明确ACE2在仔猪空肠中的分布表达,以及B^0AT1及SGLT-1和GLUT-2的mRNA在不同日龄仔猪空肠组织中表达,并通过检测血浆中游离氨基酸的种类和含量,分析各指标之间的关系。结果表明,ACE2主要分布在小肠皱襞和绒毛处,空肠上皮细胞的刷状缘、浆膜层和肌层均有ACE2的显著表达;随着仔猪日龄的增加,ACE2与B^0AT1、SGLT-1的mRNA表达量及血液中性氨基酸的含量均随之上升,GLUT-2的mRNA表达量无明显变化。结果提示,仔猪空肠中有ACE2存在,且ACE2与肠道氨基酸等物质的转运有关,这为挖掘ACE2的新功能提供了理论依据。This study was conducted to explore the correlation among the angiotensin-converting enzyme(ACE2),the neutral amino acid transporter B^0AT1 and the glucose transporters SGLT-1 and GLUT-2 by studying their distribution and expression in the intestines of piglets of different ages.Weaning piglets were used as the experiment animals.The middle jejunum tissues of piglets at 21 and 35 days of age were collected.The distribution and expression of ACE2 in the jejunum of piglets,and the expression of B^0 AT1,SGLT-1 and GLUT-2 mRNA in jejunal tissues of piglets at different days were determined by Immunohistochemistry,Western blot,real-time PCR and HPLC.By detecting the types and content of free amino acids in plasma,the relationship between these indexes was analyzed.The results showed that ACE2 was mainly located in the folds and villi of the small intestine,and was expressed significantly in the brush border,serosal layer and muscle layer of jejunal epithelial cells;As the piglets grew older,the mRNA expressions of ACE2,B^0AT1 and SGLT-1 and the content of serum neutral amino acids all increased,while the mRNA expression of GLUT-2 showed no significant change.These results indicated that ACE2 existed in jejunum of piglets,and ACE2 was related to the transport of amino acids and other substances in intestine.This fact provides the basis for the exploration of the unknown new functions of ACE2.

关 键 词：血管紧张素转换酶2 中性氨基酸转运载体 葡萄糖转运载体 断奶仔猪 物质转运 

分 类 号：S852.23[农业科学—基础兽医学]