基于SLAF-seq技术构建甘蓝型油菜Polima CMS恢复系核心种质  

Construction of core collection of polima CMS restorer lines in Brassica napus L. based on SLAF sequencing

作　　者：赵绪涛 柳海东 李开祥 贾永鹏 杜德志[1] ZHAO Xu-tao;LIU Hai-dong;LI Kai-xiang;JIA Yong-peng;DU De zhi(Academy of Agricultural and Forestry Sciences,Qinghai University,Key Laboratory of Spring Rapeseed Genetic Improvement/The Qinghai Research Branch of the National Rapeseed Genetic Improvement Center,Spring Rape Scientific Observation Experimental Station of Ministry of Agriculture and Rural Areas,Qinghai Research and Development Center for Spring Rapeseed,Xining 810016,China)

机构地区：[1]青海省农林科学院/青海大学/春油菜遗传改良重点实验室/国家油菜改良中心青海分中心/农业农村部春油菜科学观测实验站/青海省春油菜研究开发中心

出　　处：《中国油料作物学报》2019年第4期497-506,共10页Chinese Journal of Oil Crop Sciences

基　　金：现代农业产业技术体系建设专项(CARS-12);青海省春油菜遗传改良重点实验室(2017-ZJ-Y09);引进国际先进农业科学技术948项目(2014-Z58);国家重点研发计划(2018YFD0100501);国家重点研发计划(2016YFD0101304)

摘　　要：为加快选育适应春油菜区的强优势杂交种,构建甘蓝型油菜Polima CMS恢复系核心种质,以118份波里马不育系的恢复系为材料,利用简化基因组测序技术(SLAF-seq),开发SNP标记,分析群体遗传关系,结合Core Hunter软件进行核心种质构建,得到2516个有效SNP标记位点。基于有效SNP标记,对118份资源进行遗传分析,结果显示:恢复系间平均Nei’s遗传距离为0.319,含有不同遗传成分的恢复系之间遗传距离较大;118份资源被聚成5类,来源相同的材料大多被分为同一类;获得两个符合要求的核心种质子集,分别包含34份材料和46份材料,分别命名为C30和C40。通过评价这两种核心种质,等位基因覆盖度都达到了99.8%以上,MR(罗杰斯距离)和CE(卡瓦利-爱德华距离)两种遗传距离指数相差不大,且多态性含量达到了0.28,表明构建的甘蓝型油菜恢复系的核心种质可以代表118份资源,符合植物资源核心种质的构建要求。In order to provide a foundation base for conservation,selection,heterosis and hybridization as well as for utilizing germplasm,118 Brassica napus of Polima CMS restorer lines were studied and sequenced through specific length amplified fragment sequencing (SLAF-seq).By developing SNP markers and analyzing genetic relationships,core collections were conducted.A number of 2516 effective SNP loci were developed.118 Brassica napus L.restorer lines were used to construct core collection and their genetics were analyzed,based on these effective SNP markers.Results showed that the average Nei’s genetic distance between these restorer lines was 0.319,different genetic components of restore lines had a large genetic distance.118 restorer lines were clustered into five categories,most of the materials with the same origins were classified into the same category.The core collection of Brassica napus had two types of qualified varieties,containing 34 and 46 lines respectively,which named C30 and C40.By evaluating these two core collections,the allelic coverage of these two subsets was more than 99.8% and the MR (Rogers distance) and CE (Cavali-Edward distance) genetic distance indices were similar,while the polymorphic content reached 0.28.The core germplasm of the constructed Brassica napus L.restorer lines met the requirement of core collection,and could represent the original species.

关 键 词：甘蓝型油菜 波里马雄性不育恢复系 SNP分子标记 SLAF标签 核心种质 

分 类 号：S565.4[农业科学—作物学]