野生大豆转录因子GsWRKY57基因的克隆与抗旱性功能分析  

Cloning and function of GsWRKY57 transcription factor gene response to drought stress

作　　者：王岩岩 张永兴 郭葳[1] 代文君 周新安[1] 矫永庆[1] 沈欣杰[1] WANG Yan-yan;ZHANG Yong-xing;GUO Wei;DAI Wen-jun;ZHOU Xin-an;JIAO Yong-qing;SHEN Xin-jie(Oil Crop Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Wuhan 430062,China)

机构地区：[1]中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室

出　　处：《中国油料作物学报》2019年第4期524-530,共7页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31501655,31522042);中国农业科学院科技创新工程

摘　　要：WRKY转录因子是近年来在植物中发现的一类重要转录因子,在植物抗逆相关过程中发挥重要作用。本研究通过对野生大豆转录组数据进行分析,从野生大豆中克隆得到GsWRKY57基因的CDS序列。该基因开放阅读框为903bp,编码300个氨基酸残基,分子量为34.23kD,等电点为5.88。氨基酸序列分析显示该蛋白含有一个WRKY保守结构域,属于III类WRKY转录因子。系统发育树分析表明该蛋白与栽培大豆同源性最高、其次是赤豆、木豆。启动子作用元件分析预测显示该基因可能存在多种非生物胁迫作用元件。组织特异性表达分析表明该基因在大豆叶片中高丰度表达,然后依次是茎、花、荚、根。GsWRKY57基因表达受茉莉酸、水杨酸、脱落酸、干旱等植物逆境诱导。过表达GsWRKY57基因的转基因拟南芥植株相对于野生型耐旱能力增强。The WRKY transcription factor is an important transcription factor found in plants in recent years,it plays an important role in the process of plant stress resistance.In order to analyze the mechanism of GsWRKY57 transcription factors in the process of resistance response,a full –length CDS of GsWRKY57 gene was cloned by analyzing wild soybean leaf transcriptome data.Sequence analysis showed that GsWRKY57 cDNA contained 903bp ORF encoding a protein of 300 amino acids residues with molecular mass of 34.23 kD and theoretical pI of 5.88.GsWRKY57 protein contained a WRKY conservative domain structure,belonging to the class III WRKY transcription factors.Phylogenetic tree analysis demonstrated GsWRKY57 shared the highest homology with Glycine max,followed by Vigna angularis,Cajanus cajan.The promoter analyses identified the putative cis-acing regulatory elements of GsWRKY57.Tissue specific expression analysis indicated GsWRKY57 was ubiquitously expressed in leaves,stems,flowers,pods and roots,with the highest level in leaves and the lowest in root.Its expression was significantly induced by jasmonate,salicylic acid,abscisic acid and drought.Over-expression of GsWRKY57 increased drought stress tolerance in transgenic Arabidopsis lines.

关 键 词：野生大豆 GsWRKY57 转录因子 过表达 干旱胁迫 

分 类 号：S565.1[农业科学—作物学] S332.4