皖豆33对SMV株系SC3的抗性遗传分析及分子标记定位  

Inheritance and gene mapping of resistance to soybean mosaic virus strain SC3 in soybean cultivar Wandou 33

作　　者：王大刚[1] 陈圣男 黄志平[1] 于国宜 吴倩[1] 胡国玉[1] 李杰坤[1] WANG Da-gang;CHEN Sheng-nan;HUANG Zhi-ping;YU Guo-yi;WU Qian;HU Guo-yu;LI Jie-kun(Crop Institute of Anhui Academy of Agricultural Sciences/Key Laboratory of Crop Quality Improvement of Anhui Province,Hefei 230031,China;Root Biology Center,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Anhui Longkang Farm of Land-Reclamation,Bengbu 233426,China)

机构地区：[1]安徽省农业科学院作物研究所/安徽省农作物品质改良重点实验室,安徽合肥230031 [2]福建农林大学根系生物学研究中心,福建福州350002 [3]安徽农垦龙亢农场,安徽蚌埠233426

出　　处：《中国油料作物学报》2019年第4期531-536,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31571687,31201235);安徽省自然科学基金(1708085MC69);国家大豆产业技术体系建设专项(CARS-04)

摘　　要：大豆花叶病毒(soybean mosaic virus, SMV)病严重影响我国大豆产量及品质。本研究利用抗病高产稳产大豆品种皖豆33配制抗×感和抗×抗杂交组合,接种SMV优势株系SC3,研究皖豆33的抗性遗传方式及其与不同品种抗性基因间的等位性关系。结果表明:接种SC3后,抗感组合(W illiams82×皖豆33)及(皖豆33×南农1138-2)的F1单株均表现抗病,卡方测验结果显示F2群体符合3抗∶1感的分离比,F2∶ 3家系分离比符合1抗∶2分离∶1感,表明皖豆33对SC3的抗性由1对显性基因(命名为RSC3(w))控制;抗×抗组合科丰1号、齐黄1号和大白麻×皖豆33的抗性基因等位性研究显示,科丰1号与皖豆33携带对株系SC3的抗性基因是等位的或紧密连锁的,齐黄1号、大白麻与皖豆33携带抗SC3的基因是不等位且独立遗传。利用皖豆33×南农1138-2的392株F2群体将皖豆33携带SC3的抗性基因RSC3(w)定位在大豆2号染色体SSR标记BARCSOYSSR_02_0610和ZL-52之间,遗传距离分别为0.29cM和0.35 cM,物理距离约为175 kb。Soybean mosaic virus(SMV) is one of the most serious diseases to soybean yield and quality in China. In this study, the soybean resistant(R) cultivar Wandou 33 was crossed respectively with a susceptible(S) cultivar(Nannong 1138-2 or Williams 82) or a resistant cultivar(Kefeng 1, Dabaima or Qihuang 1) to determine the inheritance of Wandou 33 and test the allelism of the resistance genes when inoculated with the SMV prevalent strain SC3. The results showed that all F1 plants were resistant to SC3, the F2 plants and F2:3 progenies derived from the crosses(Williams 82×Wandou 33 and Wandou 33×Nannong 1138-2) segregated in a 3∶1(R∶S) and 1∶2∶1 ratio respectively when inoculated with SC3. This suggested that the resistance of Wandou 33 was controlled by a dominant resistance gene(named as RSC3(w)). The test of the allelism(Kefeng 1, Qihuang 1 and Dabaima×Wandou 33) of the resistance genes to SC3 indicated that the resistance genes between Kefeng 1 and Wandou 33 were alleles at a common locus or very closely linked. The resistance genes between Qihuang 1 and Wandou 33,between Dabaima and Wandou 33 were at different locus for SC3. The resistance gene RSC3(w)was located between BARCSOYSSR020610 and ZL-52 SSR markers on chromosome 2 by using 392 F2 individuals of Wandou 33×Nannong 1138-2, with a physical distance of about 175 kb. This study broadened the resources of SMV resistance genes and laid the foundation for resistance breeding.

关 键 词：大豆 大豆花叶病毒 抗性遗传 基因定位 

分 类 号：S565.1[农业科学—作物学] S432