外源二乙基二硫代氨基甲酸钠对花期淹水大豆根系抗氧化系统的影响  

Effect of exogenous sodium diethyldithiocarbamate on antioxidation system in soybean root on waterlogging at flowering stage

作　　者：齐玉军[1] 方传文 邢兴华[1] 徐泽俊[1] 王晓军[1] 孙东雷[1] 卞能飞 王幸[1] QI Yu-jun;FANG Chuan-wen;XING Xing-hua;XU Ze-Jun;WANG Xiao-jun;SUN Dong-lei;BIAN Neng-fei;WANG Xing(Jiangsu Xuhuai Regional Insititute of Agricultural Science,Xuzhou 221131,China;Qianshan Branch of Anhui Agricultural Broadcasting and Television School,Anqing 246300,China)

机构地区：[1]江苏徐淮地区徐州农业科学研究所,江苏徐州221131 [2]安徽省农业广播电视学校潜山县分校,安微安庆246300

出　　处：《中国油料作物学报》2019年第4期577-587,共11页Chinese Journal of Oil Crop Sciences

基　　金：国家现代农业产业技术体系建设专项(CARS-04);徐州市重点研发计划项目(KC18125)

摘　　要：为明确二乙基二硫代氨基甲酸钠(DDTC)对淹水胁迫下大豆根系抗氧化系统的调控作用,以耐涝性大豆品种南农1138-2和敏感性品种徐豆18为材料,通过盆栽试验,调查DDTC对花期短期(8d)淹水胁迫下大豆根系抗氧化系统的影响。结果发现:淹水胁迫下,与徐豆18相比南农1138-2根系干质量下降幅度、膜脂过氧化程度和活性氧(R OS)水平更小;同时抗坏血酸过氧化物酶(A PX)、谷胱甘肽还原酶(G R)、脱氢抗坏血酸还原酶(D HAR)活性以及还原型抗坏血酸(A sA)和还原型谷胱甘肽(G SH)含量增加幅度更大,表明南农1138-2在淹水胁迫下根系抗氧化能力更强。预喷施DDTC(在大豆初花期(R 1)叶面喷施15mmol L-1DDTC,喷施1d后进行淹水胁迫8d)能进一步提高两品种根系中过氧化物酶(POD)、 APX、DHAR和GR活性、AsA含量和AsA/DHA比值,显著降低根系ROS水平和膜脂过氧化,增加根系干重,表明DDTC通过调节抗氧化系统中抗氧化酶活性和非酶抗氧化剂含量提高了根系活性氧清除能力。To investigate the regulatory role of DDTC (sodium diethyldithiocarbamate) in antioxidant system of waterlogging stressed soybean,2 Glycine max varieties Xudou 18 (sensitive) and Nannong 1138-2 (tolerant) were used.By the mode of 1 d after spraying DDTC at flowering stage followed by 8 d waterlogging,antiosidation system changes were investigated in roots at flowering stage with pot experiments.Results showed that under the stress,Nannong 1138-2 had decreased less in root dry mass,less membrane lipid peroxidation damage,and lower ROS (reactive oxygen species) levels,but more active on APX (ascorbate peroxdidase),GR (glutathione reductase) and DHAR (dehydroascorbate reductase),and its contents of ascorbic acid (AsA) and glultathione (GSH) were increased.It indicated that Nannong 1138-2 had stronger antioxidant capacity under waterlogging.The result showed that DDTC could further increase the activities of POD (peroxidase),APX,DHAR and GR.It led to increasing AsA content and AsA/DHA ratio in roots,and to significantly reducing root ROS levels and membrane lipid peroxidation damage.It suggested that DDTC increased the ROS-scavenging activity in roots by regulating the antioxidant enzyme activity and non-enzymatic antioxidant content in antioxidant system.

关 键 词：淹水胁迫 二乙基二硫代氨基甲酸钠 大豆 花期 根系 抗氧化 

分 类 号：S565.1[农业科学—作物学]