TRECs联合CD31^+调节性T细胞检测用于分析慢性乙型肝炎患者的胸腺新近输出功能  

T cell receptor rearrangement excision circles (TRECs) and CD31^+ regulatory T cells for assessing recent thymic output in patients with chronic hepatitis B

作　　者：张永乐[1] 潘克女 施新萍[1] 王娟[1] 金鑫[1] 刘寿荣 陈科[3] Zhang Yongle;Pan Ke′nyu;Shi Xinping;Wang Juan;Jin Xin;Liu Shourong;Chen Ke(Gene Detection Department, Tongde Hospital of Zhejiang Province, Hangzhou 320012, China;Infectious Disease Department, Xixi Hospital of Hangzhou, Hangzhou 320023, China;Laboratory Medicine and Life Science of Wenzhou Medical University, Wenzhou 325035, China)

机构地区：[1]浙江省立同德医院基因检测中心,杭州320012 [2]杭州市西溪医院感染科,320023 [3]温州医科大学检验学院生命科学学院,325035

出　　处：《中华微生物学和免疫学杂志》2019年第7期514-518,共5页Chinese Journal of Microbiology and Immunology

基　　金：浙江省医药卫生科研基金项目(2017KY279).

摘　　要：目的探讨T细胞受体重排删除环(TRECs)联合CD31^+调节性T细胞(Treg)检测在评价慢性乙型肝炎(CHB)患者胸腺新近输出功能的临床应用价值。方法实验分4组:轻度慢性乙型肝炎患者(MildCHB)组35例、中度慢性乙型肝炎患者(ModerateCHB)组35例,重度慢性乙型肝炎患者(SevereCHB)组35例,健康对照(HCs)组30例,利用磁性细胞分离法分离各组CD4+CD25+Treg,流式细胞分析法检测各组外周血中CD31^+Treg占Treg细胞的比例,应用实时荧光定量PCR技术检测CD4+CD25+Treg中TRECs的数量,同时常规检测CD3^+/CD4^+/CD8^+等指标。结果ModerateCHB组和SevereCHB组外周血CD31^+Treg/Treg、TRECs的数量明显低于MildCHB组和HCs组(P<0.05);而MildCHB和HCs组间比较,差异无统计学意义(P>0.05);CD3^+/CD4^+/CD8^+在4个实验组中比较差异均无统计学意义(P>0.05)。CHB患者中CD31+Treg/Treg与TRECs的数量呈正相关(r=0.551,P=0.014)。结论ModerateCHB和SevereCHB人群中CD31^+Treg/Treg、TRECs含量减少;CD31^+Treg/Treg与TRECs含量呈正相关,是评价胸腺新近输出功能的新指标。Objective To evaluate the clinical value of combined detection of T cell receptor rearrangement excision circles (TRECs) and CD31^+ regulatory T (Treg) cells for accessing the recent thymic output in patients with chronic hepatitis B. Methods Four groups involving 135 subjects were set up in this study as follows: mild chronic hepatitis B (Mild CHB, n=35), moderate chronic hepatitis B (Moderate CHB, n=35), severe chronic hepatitis B (Severe CHB, n=35) and healthy control (HCs, n=30) groups. CD4^+ CD25^+ Treg cells in these subjects were sorted out using magnetic cell separation. The ratio of peripheral CD31+ Treg cells to Treg cells in each group was analyzed by flow cytometry. Real-time PCR was performed to detect TRECs in CD4^+ CD25^+ Treg cells. The percentages of CD3^+, CD4^+ and CD8^+ T cell subsets were also measured. Results The ratios of CD31^+ Treg/Treg cells and the numbers of TRECs in peripheral blood of the Moderate CHB and Severe CHB groups were significantly lower than those of the Mild CHB and HCs groups (P<0.05), while no statistical difference was found between the mild CHB and HC groups (P>0.05). No significant difference in the percentages of CD3^+, CD4^+ or CD8^+ T cell subsets was observed between the four groups (P>0.05). CD31^+ Treg/Treg cell ratio had a positive correlation with the number of TRECs (r=0.551, P=0.014). Conclusions Both CD31^+ Treg/Treg cell ratio and the number of TRECs were reduced in the peripheral blood of patients with moderate or severe CHB. CD31^+ Treg/Treg cell ratio and the number of TRECs were positively correlated and could be used as new indices to evaluate recent thymus output.

关 键 词：慢性乙型肝炎 CD4^+CD25^+调节性T细胞 CD31^+调节性T细胞 T细胞受体重排删除环 

分 类 号：R512.62[医药卫生—内科学]