东北地区茄子黄萎病菌致病力分化与ISSR遗传变异分析  

Pathogenicity Differentiation and ISSR Genetic Variation Analysis of Verticillium dahliae Isolates from Eggplant in Northeast China

作　　者：贲海燕[1,3] 曲红云 高苇[3] 张军民 张雪岩[1] 李宝聚[2] 柴阿丽[2] BEN Haiyan;QU Hongyun;GAO Wei;ZHANG Junmin;ZHANG Xueyan;LI Baoju;CHAI Ali(Gardening Branch,Heilongjiang Academy of Agricultural Sciences,Harbin 150069,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Institute of Plant Protection,Tianjin Academy of Agricultural Sciences,Tianjin 300381,China)

机构地区：[1]黑龙江省农业科学院园艺分院,哈尔滨150069 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]天津市农业科学院植物保护研究所,天津300381

出　　处：《园艺学报》2019年第7期1320-1332,共13页Acta Horticulturae Sinica

基　　金：公益性行业（农业）科研专项项目（201503109）;黑龙江省农业科技创新工程院级科研项目（2014ZD017）;黑龙江省农业科学院引进博士人员科研启动金项目（201507-49）;蔬菜种质创新国家重点实验室开放课题项目（201801）;中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)

摘　　要：为了揭示东北地区茄子黄萎病菌的遗传变异,从东北地区4个省49个县(市)的茄子主产区采集黄萎病菌株,从中分离、鉴定并选取80个单孢菌株,对其培养特性、致病力和ISSR(Inter-simple sequencerepeat)遗传分化进行了研究。菌株培养性状研究表明,在PDA培养基上存在4种不同的培养类型,其中菌核型菌株最多,占供试菌株的56.25%,菌丝型菌株最少,仅占供试菌株的7.5%。通过鉴别寄主接种试验,对供试菌株进行致病力分化测定,明确了东北地区茄子主产区存在黄萎病菌强(Ⅰ型)、中(Ⅱ型)、弱(Ⅲ型)3种致病力类型,分别占供试菌株的26.25%,31.25%和42.50%。特异性引物PCR检测结果显示,在供试菌株中落叶型菌株占31.25%,以中、强致病力菌株为主;非落叶型菌株为68.75%,多为弱致病力类型菌株。ISSR的聚类分析结果表明,东北地区茄子黄萎病菌的遗传分化较小,并且遗传分化与地理来源存在明显的相关性。This study revealed the genetic variation of eggplant Verticilium dahlia in Northeast China by studying the characteristics,pathogenicity and ISSR(Inter-simple sequence repeat)genetic variation of 80 representative eggplant V. dahliae strains collected from 49 major eggplant-growing counties in four northeastern provinces. The results showed that 80 tested strains were divided into 4 different culture types(A to D)according to cultural characteristics on PDA medium. Among them,sclerotia type isolates were the most(56.25% of the tested strains)and mycelium type isolates were the least(7.5% of the tested strains). The pathogenicity test showed that 80 isolates had obvious pathogenicity differentiation and could be classified into three pathogenic types,i.e. strong(Ⅰ),moderate(Ⅱ)and weak(Ⅲ),which occupied for 26.25%,31.25% and 42.50% of the total tested strains,respectively. According to the results of PCR,the deciduous isolates which accounted for 31.25% were mainly moderate and strong pathogenic strains;the non-deciduous strains which accounted for 68.75% were mostly weak pathogenic strains. ISSR analysis indicated that the genetic variation of 80 isolates was small and related to geographic region.

关 键 词：茄子 黄萎病菌 培养性状 致病力 致病类型 ISSR 

分 类 号：S641.1[农业科学—蔬菜学]