吸血库蠓胃血血源分子鉴定及吸血习性多样性研究  

Molecular identification of blood meal source in Culicoides and study on diversity of blood-sucking habit

作　　者：刘仰青[1] 陶卉英[1] 虞以新[2] 夏文[1] 马红梅[1] 柳小青[1] 陈海婴[1] LIU Yang-qing;TAO Hui-ying;YU Yi-xin;XIA Wen;MA Hong-mei;LIU Xiao-qing;CHEN Hai-ying(The Collaboration Unit for Field Epidemiology of State Key Laboratory of Infectious Disease Prevention and Control, Nanchang Center for Disease Control and Prevention, Nanchang 330038, Jiangxi Province, China;Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences)

机构地区：[1]南昌市疾病预防控制中心,传染病预防控制国家重点实验室研究基地,江西南昌330038 [2]军事医学科学院微生物流行病研究所,北京100071

出　　处：《中国媒介生物学及控制杂志》2019年第4期418-421,共4页Chinese Journal of Vector Biology and Control

基　　金：南昌市科技支撑计划(洪科字〔2018〕235号-1)~~

摘　　要：目的运用PCR技术检测吸血库蠓胃血来源,研究其吸血习性的多样性。方法 2017年8月,在南昌市郊区一村庄运用诱蚊灯法在与人类活动关系密切的生境调查吸血库蠓的组成,捕捉饱血库蠓,根据调查吸血库蠓可能的吸血对象的线粒体DNA细胞色素b序列的差异,设计特异性引物,建立PCR反应体系,设立动物血源的阳性对照和阴性对照鉴定蠓胃血来源。结果农户中以荒川库蠓和尖喙库蠓为主,分别占37.01%和33.85%;鸡圈主要以荒川库蠓为主,占81.40%;猪圈以尖喙库蠓为主,占57.66%。PCR检测库蠓胃血血源结果显示,该方法可以从已知动物血样和吸血库蠓提取的DNA中分别扩增到宿主动物的特异性条带。荒川库蠓、尖喙库蠓、刺鳌库蠓和原野库蠓均吸食人血或动物血,且4种库蠓同时具有兼吸2~4种动物血液的习性。4种不同吸血库蠓吸食人血指数荒川库蠓为0.13,尖喙库蠓为0.63,刺鳌库蠓为0.33,原野库蠓为1.00。结论建立的PCR检测方法可鉴定吸血库蠓蠓胃血来源,结果灵敏、稳定可靠;4种吸血库蠓均兼吸人、畜血液,吸血对象广泛,吸血习性多样。Objective To identify the blood meal source in Culicoides using polymerase chain reaction(PCR), and to study the diversity of blood-sucking habit. Methods Blood-engorged Culicoides were collected by light-traps in habitats related to human activities at a village in suburban Nanchang, China, August 2017. Species-specific primers were designed based on differences between mitochondrial cytochrome b sequences of common Culicoides hosts to set up a PCR system. The blood meal source in Culicoides was identified with positive and negative controls of animal blood source. Results Culicoides arakawae and C. oxystoma were the predominant species in the peasant household habitat, accounting for37.01% and 33.85%, respectively. Culicoides arakawae(81.40%) was the dominating specie in chicken coops and C. oxystoma(57.66%) was the predominant specie in pigsties. Results of PCR for blood meal source in Culicoides showed that specific PCR products were amplified from the DNA extracted from Culicoides and known animal blood samples.Moreover, C. arakawae, C. oxystoma, C. punctatus, and C. homotomus could suck both human and animal blood, and they had the habit of simultaneously sucking blood from 2-4 host animals. The blood sucking index for human blood of C. arakawae, C. oxystoma, C. punctatus, and C. homotomus was 0.13, 0.63, 0.33, and 1.00, respectively. Conclusion The PCR method has advantages in sensitivity and reliability in identifying the Culicoides blood meal source. The four Culicoides are all capable of feeding on human and animal blood, with wild blood-sucking objects and diverse habit.

关 键 词：库蠓 蠓胃血 吸血多样性 聚合酶链式反应 血源鉴定 不同生境 

分 类 号：R384.5[医药卫生—医学寄生虫学]