白纹伊蚊蛹期X射线辐照不育技术的初步实验研究  

A preliminary experimental study of X-ray-induced sterility in Aedes albopictus pupae

作　　者：邱杰 胡春明 杜文婷[1,2] 余朝举 童剑飞[1,2] 刘阳 张韶华[4] QIU Jie;HU Chun-ming;DU Wen-ting;YU Chao-ju;TONG Jian-fei;LIU Yang;ZHANG Shao-hua(Dongguan Neutron Science Center, Dongguan 523803, Guangdong Province, China;Institute of High Energy Physics, Chinese Academy of Science;University of Chinese Academy of Science;Shenzhen Center for Disease Control and Prevention)

机构地区：[1]东莞中子科学中心,广东东莞523803 [2]中国科学院高能物理研究所,北京100049 [3]中国科学院大学,北京100049 [4]深圳市疾病预防控制中心,广东深圳518055

出　　处：《中国媒介生物学及控制杂志》2019年第4期422-426,共5页Chinese Journal of Vector Biology and Control

基　　金：广东省省级科技计划项目(2015A020213001)~~

摘　　要：目的 X射线辐射昆虫不育技术是一种新型的白纹伊蚊防控手段。通过白纹伊蚊蛹期X射线系列辐照实验,可以得到X射线辐照和不育效果之间的关系,为进一步应用X射线辐射昆虫不育技术防控白纹伊蚊及其蚊媒疾病的流行奠定基础。方法利用培育自中国南方地区的白纹伊蚊蛹,进行不同发育期蛹和不同X射线辐照强度的系列实验,以考察蚊虫羽化率、存活率、生存力、不育强度以及雄性交配竞争力等因素与辐照之间的关系。采用单因素方差分析和多重比较(Tukey hoc-post test)分析各实验变量的影响。结果在<60 Gy的剂量下,X射线辐射基本不影响伊蚊蛹羽化率和辐射蛹孵化培育出成蚊的存活率,但会降低成蚊的平均寿命。在40 Gy剂量下,伊蚊蛹孵化培育的雄性成蚊接近97.0%不育,其竞争交配能力降低为0.5±0.0;在40或60Gy的辐射下,辐射蛹孵化培育的雌性成蚊则接近100%不育。释放比例为7∶1时,不育雄蚊诱导的不育率为74.1%。结论对白纹伊蚊蛹期辐照不育来说,40 Gy的X射线剂量是最佳不育剂量,验证了X射线辐射不育用于白纹伊蚊防控的可行性。Objective X-ray irradiation-based sterile insect technique(SIT) is a new technique for Aedes albopictus control. A series of X-ray irradiation experiments was performed to obtain the association between X-ray irradiation and sterility effect, in order to lay a foundation for further application of X-ray-irradiation SIT in the control of Ae. albopictus and related mosquito-borne diseases. Methods Aedes albopictus pupae in different developmental stages were collected in South China and were irradiated under different X-ray doses to evaluate the association of emergence rate, viability,survivability, degree of sterility, and male mating competitiveness with irradiation. A one-way analysis of variance and multiple comparison(Tukey hoc-post test) were used to analyze the influence of each experimental variable. Results X-ray irradiation at a dose of <60 Gy had no effect on the emergence rate of Ae. albopictus pupae and the survival rate of adult mosquitoes after hatching, but it reduced the average longevity of adult mosquitoes. At a dose of 40 Gy, the sterility rate of adult male mosquitoes reached nearly 97.0%, and male mating competitiveness was reduced to 0.5±0.0;the sterility rate of adult female mosquitoes reached almost 100%. The induced sterility rate of male mosquitoes was 74.1% at a release ratio of7∶1. Conclusion X-ray at the dose of 40 Gy is the optimal dose for induced sterility in Ae. albopictus pupae, which verifies the feasibility of X-ray irradiation-based SIT in Ae. albopictus prevention and control.

关 键 词：X射线辐照 白纹伊蚊 蛹 昆虫不育技术 

分 类 号：R384.1[医药卫生—医学寄生虫学]