谷胱甘肽复方注射液对免疫性肝纤维化大鼠肝组织钙调素表达的影响  

Study of compound glutathione inosine injection effect on expression of regucalcin in liver tissue of rats with immune hepatic fibrosis

作　　者：刘俊英[1] 刘洋[1] 李涵 时朝辉 梁祖兰[1] 王玲慧[1] 张钰[1] 赵媛 范玉梅 吴斌 于彦章[1] Liu Junying;Liu Yang;Li Han;Shi Chaohui;Liang Zulan;Wang Linghui;Zhang Yu;Zhao Yuan;Fan Yumei;Wu Bin;Yu Yanzhang(Zhoukou Central Hospital, Zhoukou 466000, China)

机构地区：[1]河南省周口市中心医院,466000

出　　处：《中华肝脏病杂志》2019年第8期628-633,共6页Chinese Journal of Hepatology

摘　　要：目的探讨免疫性肝纤维化大鼠肝组织中抗衰老标志蛋白钙调素(RGN)的表达变化,并观察谷胱甘肽复方注射液(CGII)对其的影响。方法应用猪血清腹腔注射诱导大鼠肝纤维化模型,适时给予CGII干预。取大鼠肝组织行HE及Masson染色。荧光定量PCR和免疫组化检测肝组织中RGNmRNA及蛋白质表达。计量资料采用单因素方差分析,两两比较采用LDS检验,病理学半定量结果采用秩和检验分析。结果纤维化组大鼠肝组织RGNmRNA相对表达量为82.23±15.21及蛋白表达相对表达量为12.52±3.23,较正常组RGNmRNA(176.39±11.35)及蛋白水平(59.23±9.13)明显降低(P值均<0.01);CGII干预后大鼠肝纤维化程度较纤维化组明显减轻,随着肝纤维化程度减轻,干预组RGNmRNA和蛋白相对表达量分别为168.78±21.31、46.42±4.71与纤维化组及自然恢复组比较表达均增加,差异有统计学意义(P值均<0.01);而自然恢复组大鼠肝组织RGN的mRNA和蛋白相对表达量分别为86.23±17.16、14.34±5.16,均高于模型组,但差异均无统计学意义(P>0.05)。结论RGN在猪血清所致大鼠肝纤维化肝组织表达降低,而CGII干预后随着纤维化程度的减轻,RGN的表达增加,提示该蛋白可能在肝纤维化发生发展过程中起重要作用。Objective To investigate the change in expression of anti-senescence marker protein calmodulin (RGN) in liver tissues of rats with immune hepatic fibrosis, and to observe the effect of compound glutathione inosine injection (CGII) on it. Methods Rat liver fibrosis model was induced by intraperitoneal injection of porcine serum, and CGII intervention was administered at the appropriate time. Rat liver tissues were stained with HE and Masson. RGN and protein expression at mRNA in liver tissues was detected by fluorescence quantitative PCR and immunohistochemistry. One-way Anova was used for measurement data. LDS test was used for two-way comparison, and pathological semi-quantitative results were analyzed by rank-sum test. Results The relative expression of RGN mRNA and protein in liver tissue of fibrotic rats was 82.23 ± 15.21 and 12.52 ± 3.23, respectively, which were significantly lower than that of normal rats 176.39 ± 11.35 and 59.23 ± 9.13 (P < 0.01). The degree of liver fibrosis in fibrotic rats after CGII intervention was significantly lower than fibrotic rats. The relative expression of RGN mRNA and protein in the intervention group was 168.78 ± 21.31 and 46.42 ± 4.71, respectively, which were significantly higher than fibrosis and spontaneous recovery group. The difference was statistically significant (P < 0.01). The relative expression of RGN mRNA and protein in the spontaneous recovery group was 86.23 ± 17.16 and 14.34 ± 5.16, which was higher than model group. The difference was not statistically significant (P > 0.05). Conclusion The expression of RGN in liver tissue of rats with hepatic fibrosis induced by porcine serum is decreased, while the expression of RGN increases with the decrease of fibrosis after CGII intervention, suggesting that the protein may play an important role in the development of liver fibrosis.

关 键 词：大鼠 免疫性肝纤维化 钙调素 谷胱甘肽复方注射液 

分 类 号：R285.5[医药卫生—中药学]