丙型肝炎初治患者HCV-Ab和HCV-cAg检测结果的比较研究  

Comparative study of HCV-Ab and HCV-cAg detection in patients infected with hepatitis C

作　　者：黄冠成[1] 高国生[2] 胡耀仁[3] HUANG Guan-cheng;GAO Guo-sheng;HU Yao-ren(Department of Infectious Diseases, Yuyao People's Hospital, Yuyao, Zhejiang 315400, China)

机构地区：[1]余姚市人民医院感染科,浙江余姚315400 [2]宁波市第二医院检验科,浙江宁波315010 [3]宁波市第二医院肝病科,浙江宁波315010

出　　处：《中国卫生检验杂志》2019年第15期1845-1847,共3页Chinese Journal of Health Laboratory Technology

基　　金：宁波市社发重大项目(2016C51005)

摘　　要：目的探讨丙型肝炎病毒抗体(HCV-Ab)和丙型肝炎病毒核心抗原(HCV-cAg)检测结果在丙型肝炎初治患者中的差异及临床意义。方法选择2013年1月-2017年12月在宁波市第二医院和余姚市人民医院就诊的HCV感染患者225例,分别采用RT-PCR法、基因芯片法和酶联免疫法检测HCV-RNA、基因型、HCV-Ab和HCV-cAg,对检测结果进行统计学分析。结果 225例初治患者中,以基因1型为主(55. 11%),HCV-Ab阳性率(96. 89%)显著高于HCV-cAg(38. 67%)(χ^2=174. 60,P <0. 01)。基因1型和非1型的HCV-Ab阳性率和病毒载量差异均无统计学意义(P> 0. 05),但基因1型的HCV-cAg阳性率高于基因非1型(P <0. 05)。HCV-Ab阳性率与基因型、病毒载量无关(P> 0. 05),但HCV-cAg阳性率随着病毒载量的逐渐升高而增加(P <0. 05)。HCV-Ab和HCV-cAg结果模式以HCV-Ab+/HCV-cAg+和HCV-Ab+/HCV-cAg-为主,HCV-Ab+/HCV-cAg+的基因1型比例、病毒载量均显著高于HCV-Ab+/HCV-cAg-(P <0. 05)。结论针对丙型肝炎初治患者,目前国产HCV-Ab检测试剂盒具备较好的灵敏度,而HCV-cAg试剂盒检出率偏低且受多种因素的影响。Objective To investigate the clinical significance of hepatitis C virus antibody( HCV-Ab) and hepatitis C virus core antigen( HCV-cAg) detection in patients infected with hepatitis C. Methods 225 patients infected with hepatitis C in the Second Hospital of Ningbo and Yuyao People's Hospital from January 2013 to December 2017 were enrolled and conducted for the tests of HCV-RNA,genotype,HCV-Ab and HCV-cAg by RT-PCR,gene chip and enzyme-linked immunosorbent assay respectively. Results Genotype 1 was predominant( 55. 11%) in the patients and the positive rate of HCV-Ab( 96. 89%) was significantly higher than that of HCV-cAg( 38. 67%)(χ^2= 174. 60,P 0. 01). There was no statistical significance on the difference in HCV-Ab positive rate and viral load between genotype 1 and non-genotype 1 patients( P 0. 05),but the positive rate of HCV-cAg in genotype 1 patients was higher than that in non-genotype 1 patients( P 0. 05).The positive rate of HCV-Ab was not related to genotype and viral load( P 0. 05),but the positive rate of HCV-cAg increased with the increase of viral load( P 0. 05). The result modes of HCV-Ab and HCV-cAg detection were mainly HCV-Ab +/HCV-cAg + and HCV-Ab +/HCV-cAg-. The genotype 1 ratio and viral load of HCV-Ab +/HCV-cAg +were significantly higher than those of HCV-Ab +/HCV-cAg-( P 0. 05). Conclusion Domestic HCV-Ab kits have good sensitivity for patients infected with hepatitis C,but the detection rate of HCV-cAg kits is low and affected by many factors.

关 键 词：丙型肝炎 慢性 抗体 核心抗原 

分 类 号：R512.63[医药卫生—内科学]