七氟醚对低温灌注大鼠离体心脏电生理稳定性的影响  

Effect of sevoflurane on electrophysiological stability of isolated rat hearts subjected to hypothermic perfusion

作　　者：符校魁 刘艳秋 高鸿 安丽 王贵龙 李华宇 代东君 Fu Xiaokui;Liu Yanqiu;Gao Hong;An Li;Wang Guilong;Li Huayu;Dai Dongjun(School of Anesthesiology, Guizhou Medical University, Guiyang 550004,China;Department of Anesthesiology, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004,China)

机构地区：[1]贵州医科大学麻醉学院,贵阳550004 [2]贵州医科大学附属医院麻醉科,贵阳550004

出　　处：《中华麻醉学杂志》2019年第4期440-442,共3页Chinese Journal of Anesthesiology

基　　金：贵州省科技厅联合基金(黔科合LH字[2016]7252号);贵州省大学生创新创业训练计划项目(201710660020).

摘　　要：目的评价七氟醚对低温灌注大鼠离体心脏电生理稳定性的影响。方法清洁级健康成年雄性SD大鼠,体重280~360 g,制备供体心脏离体灌注模型,取制备成功的离体心脏24个,采用随机数字表法分为4组(n=6):以37 ℃的K-H液平衡灌注15 min后,对照组(C组)、七氟醚组(S组)、32 ℃低温组(H组)和32 ℃低温+七氟醚组(HS组)分别继续灌注37 ℃ K-H液、37 ℃含2.3%七氟醚、32 ℃ K-H液和32 ℃含2.3%七氟醚的K-H液30 min。于平衡灌注15 min(T0)和继续灌注30 min(T2)时记录HR和左心室前壁三层心肌单相动作电位,计算跨室壁复极离散度(TDR),观察心律失常的发生情况。结果与C组和S组比较,H组和HS组T1时HR减慢,TDR增大,心律失常发生率升高(P<0.05);与H组比较,HS组T1时TDR减小,心律失常发生率降低(P<0.05)。结论七氟醚可改善低温灌注大鼠离体心脏电生理的不稳定性,从而降低心律失常的发生。Objective To evaluate the effect of sevoflurane on the electrophysiological stability of isolated rat hearts subjected to hypothermic perfusion. Methods Clean-grade healthy adult male Sprague-Dawley rats, weighing 280-360 g, were heparinized and anesthetized with pentobarbital sodium.Their hearts were excised and perfused in a Langendorff apparatus with K-H solution saturated with 95% O2-5% CO2 at 37 ℃.Twenty-four Langendorff-perfused hearts were divided into 4 groups (n=6 each) using a random number table method: control group (C group), sevoflurane group (S group), 32 ℃ hypothermia group (H group) and 32 ℃ hypothermia plus sevoflurane group (HS group). After 15 min of equilibration, the isolated hearts were continuously perfused for 30 min with K-H solution at 37 ℃, with K-H solution containing 2.3% sevoflurane at 37 ℃, with K-H solution at 32 ℃, and with K-H solution containing 2.3% sevoflurane at 32 ℃ in C, S, H and HS groups, respectively.Heart rate and monophasic action potential in three layers of the left ventricular anterior wall were recorded at 15 min of equilibration (T0) and 30 of continuous perfusion (T2), the transmural dispersion of repolarization (TDR) were calculated, and the occurrence of arrhythmia was observed. Results Compared with C and S groups, the heart rate was significantly decreased and TDR was enlarged at T1, and the incidence of arrhythmia was increased in H and HS groups (P<0.05). Compared with H group, TDR was significantly reduced at T1, and the incidence of arrhythmia was decreased in HS group (P<0.05). Conclusion Sevoflurane can improve the electrophysiological instability of isolated rat hearts subjected to hypothermic perfusion, and thus decrease the development of arrhythmia.

关 键 词：麻醉药 吸入 低温 心脏电生理学 

分 类 号：R614[医药卫生—麻醉学]