HPLC波长切换法同时测定八珍益母丸中5种成分含量  

Simultaneous Determination of Five Constituents in Bazhen Yimu Pills by HPLC with Wavelength Switching

作　　者：路茂菊 张娉 Lu Maoju;Zhang Ping(Aba Institute for Food and Drug Control,Sichuan Aba 624000,China)

机构地区：[1]阿坝州食品药品检验所

出　　处：《中国药师》2019年第8期1570-1573,共4页China Pharmacist

摘　　要：目的:建立HPLC波长切换法同时测定八珍益母丸中芍药苷、阿魏酸、甘草苷、甘草酸铵和毛蕊花糖苷的含量。方法:以L9(34)正交试验优化提取条件,采用Kromasil C18柱(250 mm×4.6 mm,5μm)色谱柱,以0.5%磷酸溶液(A)-乙腈(B)为流动相,梯度洗脱,流速1.0 ml·min^-1,检测波长:230 nm(芍药苷、甘草苷、甘草酸铵)、320 nm(阿魏酸、毛蕊花糖苷),柱温:30℃,进样量:10μl。结果:芍药苷、阿魏酸、甘草苷、甘草酸铵和毛蕊花糖苷的线性范围分别为4.011~64.176,1.121~17.936,1.355~21.680,0.504~8.064,3.402~54.432μg·ml^-1(r为0.999 6~0.999 9);平均加样回收率分别为99.9%,98.8%,99.2%,98.2%,98.9%(RSD<2.0%,n=6)。结论:本研究建立的方法可用于八珍益母丸的多组分同时测定。Objective: To develop a method for the simultaneous determination of peaoniflorin,ferulic acid,liquiritin,glycyrrhizic and acteoside in Bazhen Yimu pills by HPLC with wavelength switching.Methods: The L9 (34)orthogonal design was carried out in order to optimize the extraction method.The analysis was carried out on a Kromasil C18 (250 mm × 4.6 mm,5 μm) column with the mobile phase consisting of 0.5% phosphoric acid (A)-acetonitrile (B) with gradient elution at a flow rate of 1.0 ml·min^-1 .The detection wavelength was set at 230 nm for peaoniflorin,liquiritin and glycyrrhizic,and 320 nm for ferulic acid and acteoside.The column temperature was set at 30℃ and the injection volume was 10 μl.Results: The linear range was 4.011-64.176 μg·ml^-1 for peaoniflorin,1.121-17.936 μg·ml^-1 for ferulic acid,1.355-21.680 μg·ml^-1 for liquiritin,0.504-8.064 μg·ml^-1 for glycyrrhizic and 3.402-54.432 μg·ml^-1 for acteoside (r = 0.999 6-0.999 9),and the average recovery was 99.9%,98.8%,99.2%,98.2% and 98.9%(RSD < 2.0%,n = 6),respectively.Conclusion: The method can be used to determine multi-constituent simultaneously in Bazhen Yimu pills.

关 键 词：高效液相色谱法 八珍益母丸 含量测定 芍药苷 阿魏酸 甘草苷 甘草酸铵 毛蕊花糖苷 正交试验 

分 类 号：R927.2[医药卫生—药学]