SCM-198 protects endometrial stromal cells from oxidative damage through Bax/Bcl-2 and ERK signaling pathways  

作　　者：Yunyun Li Yikong Lin Xixi Huang Chunfang Xu Xinhua Liu Li Wang Min Yu Dajin Li Yizhun Zhu Meirong Du 

机构地区：[1]NHC Key Laboratory of Reproduction Regulation (Shanghai Institute of Planned Parenthood Research), Hospital of Obstetrics and Gynecology, Fudan University Shanghai Medical College, Shanghai 200011,China [2]Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases,Shanghai 200011, China [3]Shanghai Key Laboratory of Bioactive Small Molecules,Department of Pharmacology,School of Pharmacy, Fudan University,Shanghai 201203, China [4]State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy,Macao University of Science and Technology, Macao SAR, China

出　　处：《Acta Biochimica et Biophysica Sinica》2019年第6期580-587,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Basic Research Program of China (2015CB943300 and 2017YFC1001403);National Natural Science Foundation of China (81630036, 91542116,31570920,81490744,31171437,31270969,81571512,and 81501334);Innovation-oriented Science and Technology Grant from NHC Key Laboratory of Reproduction Regulation (CX2017-2);the Program of Shanghai Academic/Technology Research Leader (17XD1400900).

摘　　要：In creasi ng amounts of evidence dem on strated that accumulative reactive oxyge n species (ROS) and apoptosis of human endometrial stromal cells (ESCs) are closely associated with endometrial dysfunction induced by oxidative stress, which plays an important role in the pathological process of multiple gyn ecological and reproduction-related diseases. SCM-198, an alkaloid active component of Leonurus japonicas Houtt, has been reported to have anti-oxidative activity. However, the specific mechanisms of SCM-198 in the prevention of endometrial damage remain unknown. In the present study, we assessed the effect of SCM-198 on hydrogen peroxide (H2O2Hnduced oxidative injury in ESCs. ESCs were pretreated with SCM-198 for 4 h and then challenged with H2O2.Morphology ch a nges, apoptosis rate, and intracellular ROS producti on were measured to assess the level of oxidative injury. Flow cytometry and western blot analysis were performed to detect the expression levels of Bax, Bcl-2, active-caspase-3, and mitogen-activated protein kinases pathways. Classic inflammation cytokines were measured by real-time polymerase chain reactions. Our results showed that SCM-198 attenuated apoptosis and ROS generation of ESCs induced by H2O2. H2O2 induced the apparent apoptotic characteristics, including fragmentation of DNA, upregulation of Bax/Bcl2, activation of caspase-3, and secretion of inflammation cytokines, which were all ameliorated by SCM-198. Furthermore, H2O2-induced apoptosis-related ERK1/2 pathway activation was restrained by SCM-198 pretreatme nt. These fin dings suggested that SCM-198 could protect ESCs from oxidative injury, mainly by inhibiting oxidative stress and reducing apoptosis.

关 键 词：SCM-198 hydrogen PEROXIDE ENDOMETRIAL STROMAL cell oxidative stress 

分 类 号：Q[生物学]