能量负平衡奶牛血清的蛋白组学及生物信息学分析  

Proteomics and bioinformatics analysis of cow serum in negative energy balance

作　　者：孙书函 舒适[1] 白云龙[1] 赵畅[1] 夏成[1] 张洪友[1] 徐闯[1] SUN Shu-han;SHU Shi;BAI Yun-long;ZHAO Chang;XIA Cheng;ZHANG Hong-you;XU Chuang(College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University, Daqing,Heilongjiang163319,China)

机构地区：[1]黑龙江八一农垦大学动物科技学院

出　　处：《中国兽医学报》2019年第7期1374-1380,共7页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31372488);奶牛重要群发生产性疾病群防群控技术及产品研发与示范资助项目(GA16B202)

摘　　要：目前集约化牛场高产奶牛产后乏情率较高,严重影响奶牛繁殖性能。尽管奶牛产后乏情发生机制已有较多报道,但奶牛产后能量负平衡所致的乏情血清蛋白组学机制尚不明确。本试验在黑龙江省某集约化牛场随即采取产后14~21d奶牛,根据奶牛血液中β-羟丁酸的含量,按判定标准将试验奶牛分为能量正平衡组(NEB,n=60)和能量负平衡组(PEB,n=60),采集血清。应用iTRAQ/LC-MS/MS联用技术研究能量负平衡奶牛血清蛋白组学,获得23种差异表达蛋白。其中NEB组表达上调的有16种,分别为淀粉样结合蛋白A前体(SAA)、脂多糖结合蛋白前体(LBP)、骨膜素前体(POSTN)、Interα胰蛋白酶抑制剂重链H4前体(ITIH4)、细丝蛋白A(FLNA)、补体C2前体(C2)、蛋白酶体α亚基2型(PSMA2)、蛋白酶体亚基β1型前体(PSMB1)、蛋白酶体α亚基4型(PSMA4)、山梨醇脱氢酶(SORD)、蛋白酶体亚基α型-7(PSMA7)、血管紧张素原前体(AGT)、热休克蛋白HSP90-α(HSP90A1)、甘油醛-3-磷酸脱氢酶(GAPDH)、精氨琥珀酸裂解酶(ASL)和血红素结合蛋白前体(HPX),表达下调的有7种Cathelicidin7前体(CAMP)、转甲状腺素蛋白前体(TTR)、多聚免疫球蛋白受体前体(PIGR)、载脂蛋白F(APOF)、核糖核酸酶4前体(RNASE4)、载脂蛋白A1前蛋白(APOA1)和载脂蛋白A5前体(APOA5)。这些差异表达蛋白多与脂类代谢和蛋白酶复合体系统相关,也与和糖酵解相关的SORD和GAPDH有关。High-yield dairy cows in intensive cattle farms currently display a high rate of post-partum oestrus inhibition,which seriously affects the reproductive performance of dairy cows.Although the postpartum oestrus inhibition in dairy cows has been widely reported,its relationship with negative energy balance in postnatal dairy cows remains unclear.14-21dpost-partum cows were selected in an intensive cattle farm in Heilongjiang province.According to the content ofβ- hydroxybutyric acid in blood,the test cows were divided into energy positive balance groups (NEB,n=60)and negative energy balance group(PEB,n=60).Application of iTRAQ/LC-MS/ MS coupled technology determined energy negative balance dairy cow serum proteomics.23differentially expressed proteins were obtained.There were 16kinds of up-regulation in NEB group,including amyloid-binding protein A precursor(SAA),lipopolysaccharide binding protein precursor (LBP),periostin precursor(POSTN),and interαtrypsin inhibitor heavy chain H4.body(ITIH4), filamin A (FLNA),complement C2precursor(C2),proteasomeαsubunit type 2(PSMA2),proteasome subunitβ1 precursor (PSMB1),proteasomeαsubunit 4type (PSMA4),sorbitol dehydrogenase(SORD),proteasome subunitα-7 (PSMA7),angiotensinogen precursor(AGT), heat shock protein HSP90-α(HSP90A1),glyceraldehyde-3-Phosphate dehydrogenase(GAPDH), argininosuccinate lyase(ASL)and heme-binding protein precursor(HPX),down-regulated expression of seven cathelicidin7precursors(CAMP),transthyretin precursors(TTR ),polyimmunoglobulin receptor precursor (PIGR),apolipoprotein F (APOF),ribonuclease 4 precursor (RNASE4),apolipoprotein A1preprotein(APOA1)and apolipoprotein A5precursor(APOA5). These differentially expressed proteins are associated with lipid metabolism and protease complex systems.It is also related to SORD and GAPDH associated with glycolysis.

关 键 词：能量负平衡 蛋白组学 iTRAQ/LC-MS/MS 差异蛋白 

分 类 号：S854[农业科学—临床兽医学]