一株高产春雷霉素菌株生长特性及其培养基优化  

Growth characteristics and optimization of fermentation medium for a high-yield kasugamycin producing Streptomyces microaureus

作　　者：梁剑光 祝金山[3] 赵明霞 栗波 王立梅 Liang Jian-guang;Zhu Jin-shan;Zhao Ming-xia;Li Bo;Wang Li-mei(College of Pharmaceutical and Life Sciences, Changzhou University,Changzhou 213164;College of Biological and Food Engineering, Changshu Institute of Technology, Changshu 215500;Zhejiang Qian Jiang Biochemical Limited by Share Ltd, Haining 314400;College of Pharmacy, Suzhou University, Suzhou 215123)

机构地区：[1]常州大学制药与生命科学学院,常州213164 [2]常熟理工学院生物与食品工程学院,常熟215500 [3]浙江钱江生物化学股份有限公司,海宁314400 [4]苏州大学药学院,苏州215123

出　　处：《中国抗生素杂志》2019年第8期920-923,共4页Chinese Journal of Antibiotics

基　　金：苏州市科技项目(No.SNG201618);常熟市科技项目(No.CN201613和No.CR201704)

摘　　要：本文从一株高产春雷霉素菌株(CIT-04)出发,对其菌株生长特性(菌形、菌浓)和发酵培养基进行研究。结果表明:菌株CIT-04在种子培养基中的菌形变化随培养时间相关,种子培养33h菌浓(PMV)达25%接种时机最佳。通过对其发酵培养基成分单因素筛选和正交优化试验,确定培养基配方:黄豆饼粉4%、食用豆油2.5%、KH2PO40.05%、玉米浆0.1%、NaCl0.5%、蛋白胨0.5%、麦芽糖3%、蚕蛹粉1%、MgSO40.03%,最终高产菌株春雷霉素发酵效价可达到18780U/mL。In this paper, the growth characteristics (the mycelium morphology and pacted mycelium volume) and fermentation medium for a strain of Streptomyces microaureus CIT-04), which produced kasugamycin, were studied. The results indicated that the hypha patterns changed greatly during the cultivation with seed medium. The best inoculate time was 33h when the seed mycelial concentration was 25% PMV. The optimization of the medium was carried out by using the orthogonal analytical method, and the optimum medium components was: soybean cake powder (4%), edible soybean oil (2.5%), KH2PO4 (0.05%), corn starch (0.1%), NaCl (0.5%), peptone (0.5%), maltose (3%), silkworm chrysalis powder (1%), and MgSO4 (0.03%). Under the optimized condition, the titer of kasugamycin produced by CIT-04 was up to 18780U/mL.

关 键 词：春雷霉素 菌株特性 培养基优化 

分 类 号：R978.1[医药卫生—药品]