2016～2018年江苏省规模猪场PRRS血清学调查  

SEROLOGICAL INVESTIGATION OF PRRS IN LARGE-SCALE PIG FARMS IN JIANGSU PROVINCE

作　　者：蔡丙严 戴建华[2] 田其真[2] 郝福星[2] 范红结[1] CAI Bing-yan;DAI Jian-hua;TIAN Qi-zhen;HAO Fu-xing;FAN Hong-jie(Nanjing Agricultural University,Nanjing 210095,China;Jiangsu Agrianimal Husbandry Vocational College,Taizhou 225300,China)

机构地区：[1]南京农业大学,南京200241 [2]江苏农牧科技职业学院,泰州225300

出　　处：《中国动物传染病学报》2019年第4期92-97,共6页Chinese Journal of Animal Infectious Diseases

基　　金：江苏省农业三新工程项目(SXGC[2017]251);江苏农牧科技职业学院科研项目(NSF201703)

摘　　要：为了解2016年1月~2018年12月期间江苏省规模猪场猪繁殖与呼吸障碍综合征(porcine reproductive and respiratory syndrome,PRRS)流行动态,本研究对该省27家规模猪场不同批次送检的2062份未免疫PRRS疫苗血清样本,采用ELISA方法进行了猪繁殖与呼吸障碍综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)抗体检测,并对抗体阳性率≥80%的猪群阶段,依据PRRSV抗体S/P分布频段选取135份血清,采用RT-qPCR方法检测并统计PRRSV阳性率,进行血清学调查。结果显示,27家未免疫PRRS疫苗猪场抗体总阳性率为68.82%。不同地区抗体阳性率较高的为宿迁和南通,分别为72.04%和71.09%,连云港地区最低为58.82%。2016~2018年呈现逐年下降的趋势,从76.41%下降至57.72%。不同猪群阶段中以公猪、后备母猪、12~16周龄育肥猪和18~25周龄育肥猪抗体阳性率较高,分别为80.95%、92.86%、86.84%和98.09%。在上述4个抗体阳性率≥80%的猪群阶段,RT-qPCR检测结果显示,24份PRRSV阳性血清样本C t值与抗体S/P值高低无相关性。血清中PRRSV阳性率以后备母猪最高为20.58%,在PRRSV抗体S/P值<0.4和S/P值≥2.5异常分布频段,血清PRRSV阳性率均≥15.38%,而0.4≤S/P值<2.5正常范围内PRRSV阳性率均≤10%,差异显著,具有统计学意义(P<0.05)。表明江苏省规模化猪场不同地区、不同规模和不同阶段猪群普遍存在 PRRSV 感染,该研究结果为规模猪场PRRS的防控提供了参考。In order to understand the epidemic trend of PRRS in large-scale pig farms in Jiangsu Province.From January 2016 to December 2018,2062 serum samples of non-immunized PRRS vaccine were collected from 27 pig farms in Jiangsu Province in different batches.The PRRSV antibody were detected by ELISA.To Pig herd stage with antibody positive rate ≥80%,135 serum samples were selected according to S/P distribution frequency band.The positive rate of PRRSV were detected and counted by RT-qPCR.Serological investigation were carried out.The results showed that the total positive rate of antibodies in 27 pig farms without PRRS vaccine was 68.82%.The positive rates of antibodies in Suqian and Nantong were 72.04% and 71.09%,respectively,and the lowest was 58.82% in Lianyungang.From 2016 to 2018,it showed a decreasing trend year by year,from 76.41% to 57.72%.The antibody positive rates of boars,reserve sows,12~16 week-old fattening pigs and 18~25 week-old fattening pigs were 80.95%,92.86%,86.84% and 98.09%,respectively.The RT-qPCR test results showed that there was no correlation between the Ct of 24 PRRSV positive serum samples and the level of the antibody S/P.The highest PRRSV positive rate was 20.58% in the four stages of pigs with above 80% antibody positive rate.The positive rate of PRRSV in abnormal frequency bands of antibody S/P value < 0.4 and S/P value < 2.5 were ≥15.38%.The positive rate of PRRSV in normal range of 0.4 < S/P value < 2.5 were ≤10%,the abnormal and normal frequency bands of PRRSV antibody positive rates were significantly different (P < 0.05).The results showed that PRRSV infection was prevalent in different areas,scales and stages of largescale pig farms in Jiangsu Province.The results provided a reference for the prevention and control of PRRS in large-scale pig farms.

关 键 词：江苏省 规模猪场 PRRS 血清学调查 

分 类 号：S852.659.6[农业科学—基础兽医学]