厚竹群落孕笋成竹期内源激素动态变化与调控作用  

Dynamic changes and regulation effect of endogenous hormone in Phyllostachys edulis ‘Pachyloen’ community during the bamboo formation course

作　　者：李应兰 申展 张艳华 李苑[1] 孙娅东 黎祖尧[1,2] LI Yinglan;SHEN Zhan;ZHANG Yanhua;LI Yuan;SUN Yadong;LI Zuyao(Jiangxi Agricultural University, Nanchang 330045, Jiangxi, China;Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization, Nanchang 330045, Jiangxi, China;College of Forestry, Nanjing Forestry University, Nanjing 210037, Jiangsu, China)

机构地区：[1]江西农业大学林学院,江西南昌330045 [2]江西省竹子种质资源与利用重点实验室,江西南昌330045 [3]南京林业大学林学院,江苏南京210037

出　　处：《中南林业科技大学学报》2019年第8期55-61,共7页Journal of Central South University of Forestry & Technology

基　　金：国家科技支撑计划课题(2015BAD04B01);中央财政林业科技推广示范项目(JXTG〔2016〕11)

摘　　要：为了研究厚竹群落在孕笋成竹期内源激素的分布特征和动态变化规律,探讨内源激素及其相互作用对厚竹孕笋成竹的影响,采集不同时期的竹鞭、竹笋和母竹样品,通过酶联免疫吸附法(ELISA),测定吲哚-3-乙酸(IAA)、赤霉素(GA)、玉米素核苷(ZR)和脱落酸(ABA)的质量分数,通过方差分析和多重比较方法分析内源激素质量分数及其比值的差异。结果表明:厚竹群落中IAA和ABA质量分数显著高于ZR和GA,但IAA和ABA之间及GA和ZR之间的差异因生长系统及发育期不同而程度不同。笋芽膨大期,竹鞭、竹笋和母竹中内源激素质量分数均是ABA> IAA> GA> ZR,竹鞭中促进生长类激素偏高,ABA显著偏低,冬笋中ZR和GA质量分数占内源激素总质量分数的比例显著高于竹鞭和母竹。竹笋生长期,竹鞭和母竹中是ABA>IAA> ZR> GA,春笋中是IAA> ABA> ZR> GA;春笋中的ZR质量分数及所占比例显著高于竹鞭,ABA的质量分数及所占比例显著低于竹鞭,GA/IAA值显著高于母竹;母竹中的内源激素总量明显偏多,并且是促进生长类激素比例较高,ZR/GA值显著高于竹鞭。新竹长成期,3个生长系统中均是ABA> IAA> ZR> GA;母竹中IAA质量分数显著偏低,ABA质量分数显著偏高。在孕笋成竹过程中,母竹和竹鞭中的IAA和ABA动态变化规律刚好相反。在新竹个体发育中,GA/ABA和GA/IAA值逐渐降低,其他激素间的比值均呈抛物线变化。综合分析得出:厚竹群落中IAA和ABA的质量分数显著偏高;在孕笋成竹过程中,不同生长系统及不同内源激素的分布特征和动态变化规律不同;内源激素及相互间的协同或拮抗作用对孕笋成竹有显著调控作用,相对较高的IAA和ZR质量分数可能是竹壁增厚的原因之一。In orderto study the distribution characteristics and dynamic changes of endogenous hormones in the Phyllostachys edulis‘Pachyloen’ community, and to explore the regulation of endogenous hormones and their interactions on Ph. edulis ‘Pachyloen’during the developing process bamboo shoots, the bamboo rhizomes, bamboo shoots and maternal bamboos from different periods were collected. Bamboo samples were assayed for the mass fraction of indole-3-acetic acid(IAA), gibberellin(GA), zeatin nucleoside(ZR)and abscisic acid(ABA) by enzyme-linked immunosorbent assay(ELISA). Analysis of Variance and multiple comparison methods were used to analyze differences in endogenous hormone mass fractions and their ratios. The results showed that the IAA and ABA mass fractions in Ph. edulis ‘Pachyloen’ were significantly higher than those in GA and ZR, but the differences between IAA and ABA and between GA and ZR were different depending on the growth systems and developmental period. During the bamboo shoots sprouts period,the mass fractions of endogenous hormones in the three growth systems were all ABA > IAA > ZR > GA. The promoted hormone were high in bamboo rhizomes, and ABA was significantly lower. The ratio of ZR and GA mass fractions to total endogenous hormone mass fractions in winter shoots was significantly higher than that of bamboo rhizomes and maternal bamboos. In the bamboo shoots growing period, the mass fractions of endogenous hormones in bamboo rhizomes and maternal bamboos were all ABA > IAA > ZR > GA, and in spring shoots was IAA > ABA > ZR > GA.The ZR mass fraction and proportion in the spring shoots were significantly higher than those in the bamboo rhizomes. The mass fraction and proportion of ABA in spring shoots were significantly lower than that of bamboo rhizomes, and the ratio of GA/IAA was significantly higher than that of maternal bamboo. The total amount of endogenous hormones in maternal bamboo were significantly higher, especially the relative content of promote growth hormones were on the

关 键 词：厚竹群落 内源激素 分布特征 动态变化 调控 

分 类 号：S795[农业科学—林木遗传育种]