星星草类萌发素蛋白基因（PutGLP2）的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of GLP Family Gene PutGLP2 of Puccinellia tenuiflora

作　　者：傅文上 李波 戴绍军[1,2] 李莹[1,2] FU Wen-shang;LI Bo;DAI Shao-jun;LI Ying(Ministry of Education Alkali Soil Natural Environmental Science Center,Northeast Forestry University,Harbin Heilongjiang 150040;Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education)

机构地区：[1]东北林业大学盐碱地生物资源环境研究中心,黑龙江哈尔滨150040 [2]东北盐碱地植被恢复与重建教育部重点实验室

出　　处：《现代农业科技》2019年第16期179-180,184,共3页Modern Agricultural Science and Technology

基　　金：中央高校基本科研业务专项资金资助项目（2572018BS03;2572017ET01）

摘　　要：类萌发素蛋白是一类种子萌发相关的特异性标记,其在植物生物和非生物胁迫应答过程中起到了重要作用。本研究通过酵母cDNA文库筛选,获得全长为1065bp的PutGLP2基因,其5′非翻译区164bp,开放读码框666bp,编码221个氨基酸,3′非翻译区235bp。利用PCR技术,克隆获得PutGLP2的ORF序列。生物信息学分析发现,PutGLP2在氨基端(N端)具有一个跨膜结构域及N端信号肽,预测定位于细胞壁或胞外间质。将PutGLP2与水稻的43种GLP蛋白进行聚类分析,发现PutGLP2属于类萌发素亚家族2成员之一,并与水稻OsGLP1-1相似性最高。本研究将为丰富植物类萌发素蛋白基因信息提供参考。Germin-like proteins are a class of specific marker associated with seed germination,which play an important role in the response of biotic and abiotic stress.In this study,a full-length 1 065 bp PutGLP2 gene was isolated through screening from yeast cDNA library,The 5′-untranslated region was 164 bp,the open reading frame was 666 bp,encoding 221 amino acids,and the 3′-untranslated region was 235 bp.The ORF sequence of PutGLP2 was cloned by PCR.Bioinformatics analysis revealed that PutGLP2 has a transmembrane domain and a signal peptide at the amino terminus(N-terminus),which is predicted to be localized to the cell wall or extracellular matrix.Phylogenetic tree analysis of PutGLP2 and 43 GLP proteins in rice revealed that PutGLP2 is a member of the germin-like protein subfamily 2 and has the highest similarity with rice OsGLP1-1.This study will provide a reference for enriching the plant germination protein gene information.

关 键 词：星星草 类萌发素蛋白 基因克隆 生物信息学分析 

分 类 号：Q943.2[生物学—植物学]