Diagnosis and Control of Classical PRRSV Strain Infection in a Pig Farm  

作　　者：Ren Sufang Guo Lihui Fu Jiangong Zhang Zhaokun Li Xingguang Wu Jiaqiang Zhang Yuyu 

机构地区：[1]Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences /Shandong Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, China [2]Qixia Station of Animal Husbandry and Veterinary Medicine, Yantai 265300, China

出　　处：《Animal Husbandry and Feed Science》2019年第2期53-55,共3页动物与饲料科学（英文版）

基　　金：Supported by National Natural Science Foundation of China(31572550);Shandong Provincial Mod-ern Agricultural Industry and Technology System(SDAIT);Agricultural Science and Technology In-novation Projects of Shandong Academy of Agricultural Sciences(CXGC2016B14,CXGC2018E11,CXGC2018E10);Taishan Scholar Project

摘　　要：The suspected cases of PRRSV in a pig farm of Shandong Province were conducted pathologic autopsy, RT-PCR amplification, and sequencing and analysis of PRRSV N protein gene. The results showed that the homology of the nucleotide sequences of protein N was 99.7% between the isolate and the classical strains (VR2332 and SD1), and 93.4% between the isolate and the highly pathogenic strains (JXA1, HuN4, SX-1 and TJM). The results suggested that the virus was classical PRRSV in the pig farm. The research would provide a reference for the diagnosis and targeted therapy of classical PRRSV infection in swine production.The suspected cases of PRRSV in a pig farm of Shandong Province were conducted pathologic autopsy, RT-PCR amplification, and sequencing and analysis of PRRSV N protein gene. The results showed that the homology of the nucleotide sequences of protein N was 99.7% be-tween the isolate and the classical strains(VR2332 and SD1), and 93.4% between the isolate and the highly pathogenic strains(JXA1, HuN4, SX-1 and TJM). The results suggested that the virus was classical PRRSV in the pig farm. The research would provide a reference for the diagnosis and targeted therapy of classical PRRSV infection in swine production.

关 键 词：PRRS PRRSV CLASSICAL STRAIN PATHOLOGICAL ANATOMY RT-PCR Sequencing 

分 类 号：S[农业科学]