miR-101和RanBP9在隐睾睾丸组织中的表达及其意义  

The expression and role of miR-101 and its target gene RanBP9 in cryptorchidism testes

作　　者：程鹏[1,2,3] 黄振宇[1,2,3] 窦贤明 毛军 徐雨辰 张贤生 Cheng Peng;Huang Zhenyu;Dou Xianming(Dept of Urology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022;The Urological Institute of Anhui Medical University,Hefei 230022;Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei 230022;Dept of Urology,Anhui NO.2 Provincial Peoples's Hospital,Hefei 230041)

机构地区：[1]安徽医科大学第一附属医院泌尿外科,合肥230022 [2]安徽医科大学泌尿外科研究所,合肥230022 [3]泌尿生殖系统疾病安徽省重点实验室,合肥230022 [4]安徽省第二人民医院泌尿外科,合肥230041 [5]皖南医学院第一附属医院弋矶山医院泌尿外科,芜湖240000

出　　处：《安徽医科大学学报》2019年第9期1343-1348,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81370749)

摘　　要：目的探讨miR-101和RanBP9在隐睾睾丸组织中的表达情况及其可能作用。方法取60只雄性6周龄ICR小鼠,随机选取30只进行左侧隐睾造模手术,视为隐睾组(Cry组),右侧睾丸位于阴囊内,视为自身对照组(SC组);另外30只进行假手术后睾丸仍位于阴囊内,视为假手术组(Con组)。小鼠根据手术后时间随机分为5组,每组包含6只隐睾组小鼠和6只假手术组小鼠,分别于术后3、7、14、21、28 d处死小鼠。称量小鼠体质量、睾丸重量,睾丸进行HE染色观察组织形态学改变,qRT-PCR检测miR-101表达水平,Western blot、qRT-PCR和免疫荧光用于检测RanBP9的表达变化情况。结果与假手术组及自身右侧睾丸对照相比,隐睾组小鼠左侧睾丸在术后7 d出现结构损坏,且睾丸体质量比出现明显下降( P <0.05);miR-101在隐睾中表达增加而RanBP9的表达减少( P <0.05)。结论隐睾中miR-101表达增加而RanBP9表达量减少,这可能是隐睾中精子发生障碍的原因之一。Objective To study the role and expression of miRNA-101 (miR-101) and Ran-binding protein 9(RanBP9) in cryptorchidism testes. Methods Sixty 6-week-old male ICR mice were randomly divided into two groups. Mice in cryptorchidism group (Cry group) underwent operation to fix left testes with fat pad onto peritoneum. Mice in the control group (Con group) underwent sham operation that the left testes were kept in the scrotum. Right testes of mice in Cry group were used as self-controls (SC group). The mice were sacrificed at 3, 7, 14, 21 and 28 days after operation. The body weight and testicular weight of the mice were recorded. The histological changes were examined by HE staining. Western blot, qRT-PCR and immunofluorescence were used to detect the expression of miR-101 and RanBP9. Results The testis index of mice in cryptorchidism group was significantly decreased at 7 days after operation compared with those in SC group and Con group ( P <0.05). HE staining showed impaired testis structure in cryptorchid mice at 7 days after surgery. The expression of miR-101 was significantly increased in the cryptorchidism group while the expression of RanBP9 was decreased ( P <0.05). Conclusion The expression of miR-101 is increased in cryptorchidism while RanBP9 is decreased in cryptorchidism, suggesting that miR-101 and RanBP9 may contribute to spermatogenesis disorder in cryptorchidism.

关 键 词：miRNA-101 RanBP9 隐睾 男性不育 

分 类 号：R691.5[医药卫生—泌尿科学]