益脾活血方调控B7-H1/PD-1通路对大鼠肝癌切除术后防止复发的机制研究  

Preventive Mechanism of Yipi Huoxue Recipe on Postoperative Recurrence for Rats after Hepatectomy by Regulating B7-H1/PD-1Signal Pathway

作　　者：梁颖[1] 程钢[1] 黄邓高[1] LIANG Ying;CHENG Gang;HUANG Denggao(The People's Hospital of Haikou City (HaikouHainan 570208,China))

机构地区：[1]海口市人民医院

出　　处：《四川中医》2019年第8期24-28,共5页Journal of Sichuan of Traditional Chinese Medicine

基　　金：海南省卫生厅项目(编号:琼卫2011~67)

摘　　要：目的:研究益脾活血方调控细胞程序性死亡-配体1(B7-H1)与细胞程序性死亡受体~1(PD-1)结合通路对大鼠肝癌切除术后防止复发的机制。方法:选用健康SPF级雄性SD大鼠36只,将其随机分为对照组、西药组、中药组,每组12只,三组接种人肝癌细胞,7天后取肝脏已成瘤裸鼠进行肝癌手术切除建立肝癌术后裸鼠模型,分别给予益脾活血方和5-氟尿嘧啶进行干预,连续4周。观察并记录裸鼠一般情况、采用PET/CT影像学检查术后复发情况、苏木精-伊红染色法(HE法)检验术后复发组织病理变化、蛋白质印迹法(WB法)检测B7-H1/PD-1表达量、流式细胞术检测CD4^+、CD8^+T淋巴细胞水平。结果:对照组、西药组、中药组三组均可在裸鼠左中上腹发现高摄取区域,西药组和中药组复发率远远低于对照组(P<0.05),西药组和中药组复发率无显著差异(P>0.05),与对照组相比,西药组和中药组B7-H1相对表达量显著降低(P<0.05),西药组B7-H1相对表达量低于中药组(P<0.05),与对照组相比,中药组和西药组术后复发裸鼠CD4^+、CD4^+/CD8^+在外周血、脾脏中显著上升(P<0.05),CD8^+细胞水平明显降低(P<0.05);西药组在外周血和脾脏中CD4^+水平显著高于中药组(P<0.05),外周血中中药组和西医组CD8^+水平比较无明显差异(P>0.05)。结论:益脾活血方可通过介导B7-H1/PD-1改善T淋巴细胞亚群失衡防止肝癌术后复发。Objective:To investigate the preventive mechanism of Yipi Huoxue Recipe on postoperative recurrence for rats after hepatectomy by regulating B7 homolog 1(B7-H1)/programmed death-1(PD-1)signal pathway.Methods:36 healthy SPF male Sprague-Dawley rats were randomly divided into controlled group,western medicine group and Chinese medicine group,with 12 rats in each group.Rats were made into models and received the corresponding treatments for 4 weeks.The general conditions,and various indicators were observed by various methods,including the recurrence examined by PET/CT imaging,the pathological changes of recurrence tissues examined by hematoxylin-eosin staining(HE),the expression of B7-H1/PD-1 detected by Western blotting(WB),and the levels of CD4^+,CD8^+T lymphocyte detected by flow cytometry.Results:The high-intake area was found in the left middle and upper abdomen of the nude mice in all three groups.The recurrence rate of the western medicine group and the Chinese medicine group was much lower than that of the controlled group(P<0.05),with no statistic difference between the western medicine group and the Chinese medicine group(P>0.05).The relative expression level of B7-H1 ranking in a descending order of the three groups was the controlled group,the Chinese medicine group and the western medicine group(P<0.05).Compared with the controlled group,CD4^+and CD4^+/CD8^+in the peripheral blood and spleen of the relapsed nude mice in the Chinese medicine group and the western medicine group increased significantly(P<0.05),and the CD8^+cell level decreased significantly(P<0.05).The CD4^+level in the peripheral blood and spleen of the western medicine group was significantly higher than that of the traditional Chinese medicine group(P<0.05).The CD8^+level in the peripheral blood between the traditional Chinese medicine group and the western medicine group had no difference(P>0.05).Conclusion:Yipi Huoxue Recipe can ameliorate the recurrence of liver cancer by mediated B7-H1/PD-1 to improve the imbalance of T lymphocyte

关 键 词：益脾活血方 B7-H1/PD-1 信号通路 术后复发 

分 类 号：R285.5[医药卫生—中药学]