Exploration of the key microbes and composition stability of microbial consortium GF-20 with efficiently decomposes corn stover at low temperatures  

作　　者：YU Xiao-fang BORJIGIN Qinggeer GAO Ju-lin WANG Zhi-gang HU Shu-ping BORJIGIN Naoganchaolu WANG Zhen SUN Ji-ying HAN Sheng-cai 

机构地区：[1]Agricultural College, Inner Mongolia Agricultural University

出　　处：《Journal of Integrative Agriculture》2019年第8期1893-1904,共12页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31760353 and 31560360);the National Key R&D Program of China (2017YFD0300804 and 2016YFD0300103);the earmarked fund for China Agriculture Research System (CARS-02-63);the Crop Science Observation & Experiment Station in Loess Plateau of North China, Ministry of Agriculture, China (25204120);the Advanced Talented Scholars of Inner Mongolia Agricultural University, China (NDYB2016-15)

摘　　要：The microbial consortium GF-20(GF-20) can efficiently decompose corn stover at low temperatures. The present study explored the key microbes of GF-20 and evaluated different culture conditions on its composition stability to promote the utilization of corn stover decomposing microbes in low temperature regions. GF-20 was subcultured to the 15 th generation under different temperatures, pHs, carbon, and nitrogen sources. Then, the dynamics of fermenting pH, cellulose enzyme activities, carbohydrate concentration, and oxidation reduction potential were determined to estimate the degradation efficiency of corn stover with GF-20. Furthermore, the structural stability and functional microbes of GF-20 were identified on the basis of PCR-denaturing gradient gel electrophoresis(DGGE) profiling and principal component analysis. The results showed that the offspring of GF-20 subcultured under different temperatures(4–30°C) and pH(6.0–9.0) conditions maintained stable growth, decomposition function, and composition structure. Furthermore, consortia GF-20 had a stable composition structure, which induced GF-20 to secrete cellulose and promote substrate decomposition as corn stover and ammonium were used as sources of carbon and nitrogen, respectively. According to the PCR-DGGE profiles, the key strains of GF-20 were determined to be Bacillus licheniformis, Cellvibrio mixtus subsp. mixtus, Bacillus tequilensis, Clostridium populeti, and Clostridium xylanolyticum.The microbial consortium GF-20(GF-20) can efficiently decompose corn stover at low temperatures. The present study explored the key microbes of GF-20 and evaluated different culture conditions on its composition stability to promote the utilization of corn stover decomposing microbes in low temperature regions. GF-20 was subcultured to the 15 th generation under different temperatures, pHs, carbon, and nitrogen sources. Then, the dynamics of fermenting pH, cellulose enzyme activities, carbohydrate concentration, and oxidation reduction potential were determined to estimate the degradation efficiency of corn stover with GF-20. Furthermore, the structural stability and functional microbes of GF-20 were identified on the basis of PCR-denaturing gradient gel electrophoresis(DGGE) profiling and principal component analysis. The results showed that the offspring of GF-20 subcultured under different temperatures(4–30°C) and pH(6.0–9.0) conditions maintained stable growth, decomposition function, and composition structure. Furthermore, consortia GF-20 had a stable composition structure, which induced GF-20 to secrete cellulose and promote substrate decomposition as corn stover and ammonium were used as sources of carbon and nitrogen, respectively. According to the PCR-DGGE profiles, the key strains of GF-20 were determined to be Bacillus licheniformis, Cellvibrio mixtus subsp. mixtus, Bacillus tequilensis, Clostridium populeti, and Clostridium xylanolyticum.

关 键 词：MICROBIAL CONSORTIUM DECOMPOSITION of CORN stalks low temperature functional MICROBE structure STABILITY 

分 类 号：S[农业科学]