Isolation and identification of Fusarium oxysporum f. sp. cubense in Fujian Province, China  

作　　者：WU Kai-li CHEN Wei-zhong YANG Shuai WEN Ya ZHENG Yu-ru Wilfred Mabeche Anjago YUN Ying-zi WANG Zong-hua 

机构地区：[1]State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China [2]Fujian Institute for Food and Drug Quality Control, Fuzhou 350001, P.R.China [3]Institute of Oceanography, Minjiang University, Fuzhou 350108, P.R.China

出　　处：《Journal of Integrative Agriculture》2019年第8期1905-1913,共9页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31601583);the Natural Science Foundation of Fujian Province, China (2016J01113);the Young Teacher Education Research Project of Fujian Province, China (JAT160178);the Fujian Agriculture and Forestry University Outstanding Youth Scientific Research Project, China (xjq201625)

摘　　要：Fusarium wilt, caused by Fusarium oxyporum f. sp. cubense(Foc), is the most serious disease affecting banana production.To clarify the distribution of the Foc races in Fujian Province of China, 79 soil samples were collected from four regions of Zhangzhou City, the primary banana production area in Fujian. We isolated and identified 12 Foc strains based on internal transcribed spacer(ITS) sequence analysis, PCR amplification by using Foc-specific primers and pathogenicity assays.Our analysis indicated that 11 isolates belong to Foc race 1, and 1 isolate belongs to the Foc tropical species race 4(TR4).Although TR4 has previously been reported to occur in primary banana-producing provinces, such as Hainan, Guangxi,and Guangdong of China, this is the first report of TR4 isolated from the soil in Fujian Province. Monitoring the presence of Foc, in particular, the TR4 strains in the soil, is the basic strategy to prevent and control Fusarium wilt.Fusarium wilt, caused by Fusarium oxyporum f. sp. cubense(Foc), is the most serious disease affecting banana production.To clarify the distribution of the Foc races in Fujian Province of China, 79 soil samples were collected from four regions of Zhangzhou City, the primary banana production area in Fujian. We isolated and identified 12 Foc strains based on internal transcribed spacer(ITS) sequence analysis, PCR amplification by using Foc-specific primers and pathogenicity assays.Our analysis indicated that 11 isolates belong to Foc race 1, and 1 isolate belongs to the Foc tropical species race 4(TR4).Although TR4 has previously been reported to occur in primary banana-producing provinces, such as Hainan, Guangxi,and Guangdong of China, this is the first report of TR4 isolated from the soil in Fujian Province. Monitoring the presence of Foc, in particular, the TR4 strains in the soil, is the basic strategy to prevent and control Fusarium wilt.

关 键 词：FUSARIUM OXYSPORUM f. sp. cubense(Foc) identification of PHYSIOLOGICAL RACES PATHOGENICITY test Fujian FUSARIUM WILT 

分 类 号：S[农业科学]