人体肱骨干显微骨硬度分布特征的实验研究  

Micro-hardness distribution of humeral shaft in human skeleton

作　　者：张晓娟[1,2,3] 李升 吴卫卫[1,2,3] 刘国彬 王建朝[1,2,3] 殷兵 胡祖圣[1,2,3] 付蕾 张英泽[1,2,3] Zhang Xiaojuan;Li Sheng;Wu Weiwei;Liu Guobin;Wang Jianzhao;Yin Bing;Hu Zusheng;Fu Lei;Zhang Yingze(Department of Orthopaedic Surgery,the Third Hospital of Hebei Medical University,Shijiazhuang 050051;Orthopaedic Research Institution of Hebei Province,Shijiazhuang 050051;Key Laboratory of Biomechanics of Hebei Province,Shijiazhuang 050051)

机构地区：[1]河北医科大学第三医院创伤急救中心,石家庄050051 [2]河北省骨科研究所,石家庄050051 [3]河北省骨科生物力学重点实验室,石家庄050051

出　　处：《中华解剖与临床杂志》2019年第4期318-321,共4页Chinese Journal of Anatomy and Clinics

基　　金：国家自然科学基金(81572125、81501934).

摘　　要：目的测量并分析肱骨干不同层面和方位显微骨硬度分布特征及其临床意义.方法选取新鲜成人尸体标本3具(62岁男性、45岁女性和58岁男性),取其右侧肱骨干部分,垂直于肱骨干长轴水平,使用金刚石慢速锯在每一段精确切取厚3 mm的骨组织标本共7层,分别标记为第Ⅰ~Ⅶ层,进而区分为肱骨干上段(Ⅰ、Ⅱ层)、中段(Ⅲ~Ⅴ层)、下段(Ⅵ、Ⅶ层),并将每层划分为前、后、内、外四个区域;应用维氏金刚石显微硬度压头在标本表面进行硬度测量并分区统计,进而分析不同区域肱骨干显微硬度分布规律.结果共获得肱骨干84个测量部位420个测量位点的有效测量值.分析显示,肱骨干总体硬度(47.52±6.01)HV,肱骨干中段整体硬度大于肱骨干上段及下段(F=11.594,P<0.01).肱骨干7个分层中,Ⅳ层硬度最大(51.34±7.01)HV、Ⅶ硬度最小(45.72±6.25)HV;肱骨中段Ⅳ层内侧部分硬度最大(53.77±8.70)HV,肱骨干下段Ⅶ层后侧区域硬度最小(42.02±7.47)HV.肱骨干后侧整体硬度(45.28±6.47)HV,小于肱骨外侧(49.12±5.22)HV、内侧(48.28±6.10)HV、前侧(47.40±5.55)HV硬度,差异有统计学意义(F=8.347,P<0.01).结论肱骨干骨皮质不同层面及方位骨显微硬度存在差异,该分布规律数据可指导设计适应生理状态下骨骼应力传导特性的3D打印仿生内置入物.亦可对模拟生理状态下骨骼生物力学性能的骨骼建模及有限元分析提供数据支持.Objective To measure and analyze the distribution and significance of bone hardness at different levels and directions of humerus shaft.Methods Three right humeral shafts from fresh cadaver specimens were included in this study,and were divided into 7 sections perpendicular to the long axis of the humeral shaft.Then,7 layers(3 mm specimen)were cut with diamond saw,and then divided into humerus upper segment(Ⅰ,Ⅱlayer),middle segment(Ⅲ-Ⅴlayer),lower segment(Ⅵ,Ⅶlayer),and each layer was divided into anterior,posterior,medial and lateral four areas.Vickers microhardness diamond pressure head was used in the specimen surface to achieve hardness measurement.The distribution of hardness was recorded and analyzed.Results A total of 84 parts of humeral shaft were measured and 420 measurements were made.The average hardness of humeral shaft was(47.52±6.01)HV.The overall hardness of the middle humeral shaft was greater than that of the upper and lower humeral shaft(F=11.594,P<0.01).Ⅳlevel of 7 horizontal layers had the maximum hardness(51.34±7.01)HV.Minimum hardness wasⅦlevel(45.72±6.25)HV.The medial part of middle shaftⅣhardness was biggest(53.77±8.70)HV,and the minimum hardness occurred in posterior part of shaft sectionⅦ(42.02±7.47 mm)HV.The overall hardness of the posterior humerus(45.28±6.47)HV was lower than that of the lateral humerus(49.12±5.22)HV,the medial humerus(48.28±6.10)HV,and the anterior humerus(47.40±5.55)HV(F=8.347,P<0.01).Conclusions There are differences in the micro-hardness of the humerus diaphyseal cortex at different levels and in the orientation of the bone.The data of this distribution can be used to guide the design of 3D-printed implants adapted to the characteristics of bone stress conduction under physiological conditions.It can also provide data support for bone modeling and finite element analysis of bone biomechanical properties under simulated physiological state.

关 键 词：肱骨干 骨硬度实验 维氏硬度 显微硬度 人体骨骼 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学]