基于NMR指导的六神曲成分研究  

NMR-based study of chemical constituents of Massa Medicata Fermentata

作　　者：张慧茹 张婷婷[1,2] 许枬 付鸣[1] 李泽煜 ZHANG Hui-ru;ZHANG Ting-ting;XU Nan;FU Ming;LI Ze-yu(School of pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Jinzhou Central Hospital,Jinzhou 121000,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]锦州市中心医院,辽宁锦州121000

出　　处：《中草药》2019年第16期3764-3768,共5页Chinese Traditional and Herbal Drugs

基　　金：2015年国家公益性行业专项课题(201507009-03)

摘　　要：目的研究六神曲的化学成分。方法运用各种色谱技术分离六神曲的化学成分,并利用理化性质和波谱技术鉴定其结构。结果从六神曲中分离得到11个成分,分别鉴定为5-二十五烷基间苯二酚(1)、5-十七烷基间苯二酚(2)、5-二十一烷基间苯二酚(3)、5-二十三烷基间苯二酚(4)、(9S,10E,12Z)-9-hydroxy-10,12-octadecadienoic acid(5)、trans-4-hydroxy-2-nonenoic acid(6)、9,10,11-trihydroxy-11(E)-octadecenoic acid(7)、(9S,12R,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid(8)、(9S,12S,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid(9)、(9S,12S,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid methyl ester(10)、9,12(Z)-octadecadienoic acid methyl ester(11)。结论化合物1~11均为首次从六神曲中分离得到。抗炎活性研究显示,六神曲氯仿提取物和化合物8均显示出良好的抗炎活性。Objective To study the chemical constituents from Medicated Leaven(namely Massa Medicata Fermentata in Chinese).Methods Compounds were isolated and purified from chloroform extract of Medicated Leaven by column chromatograph of silica gel,ODS,Sephadex LH-20,HPLC,and their structure were elucidated on the basis of spectral data and physicochemical property.Results Eleven chemical compounds were identified as 5-pentacosyl resorcinol(1),5-heptadecyl resorcinol(2),5-heneicosyl resorcinol(3),5-tricosyl resorcinol(4),(9S,10E,12Z)-9-hydroxy-10,12-octadeca-dienoic acid(5),trans-4-hydroxy-2-nonenoic acid(6),9,10,11-trihydroxy-11(E)-octadecenoic acid(7),(9S,12R,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid(8),(9S,12S,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid(9),(9S,12S,13S)-9,12,13-trihydroxy-10(E)-octadecenoic acid methyl ester(10),and 9,12(Z)-octadecadienoic acid methyl ester(11).Conclusion All above compounds are all isolated from Massa Medicata Fermentata for the first time,and the chloroform extract of Massa Medicata Fermentata and compound 8 showed inhibitory effect against TNF-αgeneration of RAW 264.7 induced by LPS in a dose-dependent manner.

关 键 词：六神曲 5-二十五烷基间苯二酚 5-十七烷基间苯二酚 三羟基-十八碳烯酸 (9S 12R 13S)-9 12 13-trihydroxy-10(E)-octadecaenoic ACID 

分 类 号：R284.1[医药卫生—中药学]