基于高通量测序技术分析2种菌草根际土壤细菌群落多样性  

Rhizosphere Soil Bacterial Community of Juncao by High-throughput Sequencing Techniques

作　　者：叶文雨[1,2] 廖海萍 许钰滢 谢序泽 倪明月 胡红莉[3] 余文英[2] 鲁国东[1,3] YE Wenyu;LIAO Haiping;XU Yuying;XIE Xuze;NI Mingyue;HU Hongli;YU Wenying;LU Guodong(National Engineering Research Center of Juncao Technology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,福建福州350002 [2]福建农林大学生命科学学院,福建福州350002 [3]福建农林大学植物保护学院,福建福州350002

出　　处：《热带作物学报》2019年第9期1783-1788,共6页Chinese Journal of Tropical Crops

基　　金：中央引导地方科技发展专项资金项目(No.2018L3003);农业引导性(重点)项目(No.2017N0005);福建省自然科学基金项目(No.2015J0504)

摘　　要：为详细了解福州菌草基地巨菌草和绿洲一号2种菌草的根际土壤细菌群落组成与结构,本研究应用高通量测序技术,分析了巨菌草和绿洲一号根际土壤细菌群落结构及多样性。结果表明,菌草根际土检测到细菌类群主要的优势菌门为酸杆菌门(Acidobacteria)、变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)、绿弯菌门(Chloroflexi)、芽单胞菌门(Gemmatimonadetes)、硝化螺旋门(Nitrospirae)、疣微菌门(Verrucomicrobia)、浮霉菌门(Planctomycetes);主要的优势菌纲为γ-变形菌纲(Gammaproteobacteria)、α-变形菌纲(Alphaproteobacteria)、芽孢杆菌纲(Bacilli)、放线菌纲(Actinobacteria)、酸杆菌纲(Acidobacteria-6)、β-变形菌纲(Betaproteobacteria)、酸杆菌纲(Acidobacteriia)、纤线杆菌纲(Ktedonobacteria)、δ-变形菌纲(Deltaproteobacteria);主要的优势属为葡萄球菌属(Staphylococcus)、糖多孢菌属(Saccharopolyspora)、类芽孢杆菌属(Paenibacillus)、沙雷氏菌属(Serratia)、链球菌属(Streptococcus)、红游动菌属(Rhodoplanes)、不动细菌属(Acinetobacter)、假单胞菌属(Pseudomonas)。绿洲一号、巨菌草及其对照根际土壤细菌Shannon-Wiener指数分别为9.05、8.733、7.61;巨菌草、绿洲一号及其对照根际土壤细菌Simpson多样性指数分别为0.01、0.014、0.037;巨菌草、绿洲一号及其对照根际土壤细菌Chao1指数分别为3145.320、3647.482、2329.344。Alpha多样性指数分析得出,在3个土壤样品中,绿洲一号根际土Shannon指数最大,对照土壤Shannon指数最小,对照土壤Simpson指数最大,绿洲一号Simpson指数最小,说明菌草根际土壤细菌群落多样性高于非根际土土壤细菌群落多样性,绿洲一号根际土土壤细菌群落多样性略高于巨菌草根际土壤细菌群落多样性,这些结果为进一步研究土壤微生物提供了理论基础。Diversity analyses were investigated by the high-throughput sequencing to study the composition and diversity of bacterial communities in the rhizosphere soil of Pennisetum giganteum and Arundo donax cv. Lvzhou No. 1. Results showed that the dominant phyla of all samples were Acidobacteria,Proteobacteria,Actinobacteria,Firmicutes, Chloroflexi,Gemmatimonadetes,Nitrospirae,Verrucomicrobia,Planctomycetes. At the class level,nine dominant species were found in all samples,such as Gammaproteobacteria,Alphaproteobacteria,Bacilli,Actinobacteria,Acidobacteria- 6,Betaproteobacteria,Acidobacteriia,Ktedonobacteria,Deltaproteobacteria. At the genus level,dominant species were found in all samples,such as Staphylococcus,Saccharopolyspora,Paenibacillus,Serratia,Streptococcus,Rhodoplanes, Acinetobacter,Pseudomonas. The Shannon-Wiener from the samples was 9.05,8.733,7.61,respectively. The Simpson index from the samples was 0.01,0.014,0.037,respectively. The Chao1 index from the samples was 3145.320, 3647.482,2329.344,respectively. The result of Alpha diversity analysis showed that the Shannon index,Simpson index and Chao1 index of Juncao increased. The diversity index analysis showed that in the soil samples,the lowest bacterial diversity was in the experimental control soil and the highest bacterial diversity was in the A. donax cv. Lvzhou No. 1 rhizosphere soil. The bacterial diversity in the A. donax cv. Lvzhou No. 1 rhizosphere soil was slightly higher than that in the Pennisetum giganteum rhizosphere soil.

关 键 词：菌草 根际土壤 细菌群落多样性 高通量测序 

分 类 号：S154.3[农业科学—土壤学] S54[农业科学—农业基础科学]