Expression and Enzyme Activity Determination of Apripona Germari Cellulase in Bmnpv/Bac-to-Bac Baculovirus Expression Systems  

作　　者：Lu Gao Zhe Yang Keping Chen 

机构地区：[1]School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu Province [2]Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu Province

出　　处：《Biomed Communication》2018年第1期1-6,共6页生物医学通讯

基　　金：the Research Innovation Plan of Graduate Students in Jiangsu Province (CX07B_09x) and (CX08B_09x);the National Natural Science Foundation of China (Grant No. 1721280032).

摘　　要：To Search for a more efficient way of degrading cellulose. METHODS: Apripona germari Cellulase genes were cloned and expressed in Bombyx mori derived cell line, BmN cell line by BmNPV/Bac-to-Bac baculovirus expression systems. Infected BmN cells were harvested 72 h post-infection (hpi). Expressd recombinant cellulose was analyzed in 12.5% SDS– PAGE and western blot. The activity of purified recombinant cellulose was assessed by 3, 5- nitro salicylic acid (DNS) colorimetric method. RESULTS: Western blot analysis showed that Recombinant cellulase was expressed in BmN insect cells as a 29 kDa protein. Enzyme activity assay demonstrated that recombinant cellulose has the activity of decomposing cellulose. CONCLUSION: Apripona germari Cellulase was successfully expressed in BmN insect cell line. The method established in this study provides an efficient way to produce a large amount of cellulase and paves the way for further utilization of cellulose.

关 键 词：Apripona Germari CELLULASE BAC-TO-BAC BACULOVIRUS Expressio ENZYME ACTIVITY 

分 类 号：R[医药卫生]