黄瓜灰霉病的生防木霉菌株筛选  

Screening of Trichoderma Strains for Biocontrol of Cucumber Gray Mold

作　　者：陈晋 郭玉杰[2] 吕恒[2] 牛永春[2] 邓晖[2] 师迎春[3] 余知和 Chen Jin;Guo Yujie;Lv Heng;Niu Yongchun;Deng Hui;Shi Yingchun;Yu Zhihe(College of Life Sciences, Yangtze University, Jingzhou 434025, Hubei;Institute of Agricultural Resources Regionalization , Chinese Academy of Agricultural Sciences, Beijing 100081;Beijing Plant Protection Station,Beijing 100029)

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]中国农科院农业资源与农业区划研究所,北京100081 [3]北京市植物保护站,北京100029

出　　处：《长江大学学报（自然科学版）》2019年第8期71-76,I0005,共7页Journal of Yangtze University(Natural Science Edition)

基　　金：中国农业科学院基本科研业务费专项(1610132018011)

摘　　要：木霉属(Trichoderma)真菌资源丰富,具有重要的生防潜力。初步研究了实验室保藏的247个木霉菌株对灰葡萄孢菌(Botrytis cinerea)的抑菌效果,对峙培养结果表明,69个木霉菌株对灰葡萄孢菌的拮抗指数大于80%。利用部分拮抗效果稳定的木霉菌株进行非挥发性物质筛选试验,其中12个菌株对 B.cinerea 表现出明显的抑制作用。离体叶片和果实的抑病试验表明,菌株M13033-1表现出显著抑制黄瓜(Cucumis sativus)灰霉病作用, TEF1-α基因和 RPB2 基因的系统发育分析显示,该菌株为非洲哈茨木霉(Trichoderma afroharzianum)。Trichoderma is rich in fungal resources and has important biocontrol potential.The inhibition effect of 247 strains of Trichoderma preserved in the laboratory against Botrytis cinerea is studied.The results of confrontation culture show that the antagonistic index of 69 Trichoderma strains against Botrytis cinereais more than 80%.The screening test of non-volatile substances is carried out on some selected strains of Trichoderma with stable antagonistic effect.The results indicate that 12 of them shows obvious inhibition to B.cinerea.The leaf and fruit experiments in vitro show that the strain M13033-1 significantly inhibites grey mold in cucumber(Cucumis sativus).Phylogenetic analysis based on sequences of TEF1-α and RPB2 genes show that M13033-1 is Trichoderma afroharzianum.

关 键 词：木霉属 (Trichoderma) 灰葡萄孢菌(Botrytis cinerea) 黄瓜(Cucumis sativus) 拮抗指数 抑病 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治]