五味子水提物抑菌活性的研究  

Study on Antimicrobial Activity of Aqueous Extract of Schisandra chinensis

作　　者：刘月月[1] 殷斌[1] 林树乾[1] 李桂明[1] 赵增成[1] 黄中利[1] 傅剑[1] 杨世发[1] Liu Yueyue;Yin Bin;Lin Shuqian;Li Guiming;Zhao Zengcheng;Huang Zhongli;Fu Jian;Yang Shifa(Poultry Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250023,China)

机构地区：[1]山东省农业科学院家禽研究所

出　　处：《山东农业科学》2019年第8期118-122,共5页Shandong Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFC1702700);山东省自然科学基金项目(ZR2019MC022);家禽研究所学科团队建设项目(CXGC2018E11);山东省现代农业产业技术体家禽首席岗位专家项目(SDAIT-13-011-01);山东省农业科学院农业科技创新工程项目(CXGC2016A10);中央引导地方科技发展专项资金(鲁财教指[2017]77号)

摘　　要：采用水煎法制备五味子水提物(SCD),通过SCD的最小抑菌浓度、最小杀菌浓度检测和纸片扩散抑制试验评估SCD的体外抑菌活性,通过SCD对雏鸡感染大肠杆菌的治疗试验评估SCD的体内抑菌活性。结果显示,SCD对6株标准株和6株大肠杆菌分离株均具有显著的抑菌活性。SCD对雏鸡感染大肠杆菌的治疗试验结果显示,口服SCD可以显著降低感染大肠杆菌雏鸡的死亡率。这表明SCD具有良好的抑菌活性,且对环丙沙星耐药的大肠杆菌均有效,可以作为治疗细菌性疾病的备选药物。The aqueous extract of Schisandra chinensis (SCD) was prepared by water decoction, and the antimicrobial activity of SCD in vitro was assessed through minimal inhibitory concentration, minimal bactericidal concentration test and Kirby-Bauer zone of inhibition assay. In addition, the antimicrobial activity of SCD in vivo was tested through therapeutic effect of oral SCD on chickens infected Escherichia coli. The results showed that SCD exhibited excellent antimicrobial activity against all the six reference bacterial strains and six field E. coli strains. Therapeutic experiment found oral SCD could significantly reduce the mortality of chickens infected by E. coli. It suggested that SCD could serve as an excellent antibacterial agent for treating bacterial infections, including CIP-resistant E. coli strains.

关 键 词：五味子 水提物 抗菌活性 耐药大肠杆菌 SPF鸡 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]