基于ERK表达探讨针刺干预大型实验动物猪心肌缺血效应的初步观察  

Preliminary Observation on the Effects of Acupuncture Intervention in Large Experimental Pigs of Myocardial Ischemia Based on ERK Expression

作　　者：张金铃[1] 李亮[1] 荣培晶[1] 孟宏[2] 田毅 何勋[1] 贾术永[1] 张今朝 李少源[1] 赵斌 周晟芳[4] 刘欢[4] 孙铭鸿 张峰 雷洪涛[5] 方继良[4] Zhang Jinling;Li Liang;Rong Peijing;Meng Hong;Tian Yi;He Xun;Jia Shuyong;Zhang Jinchao;Li Shaoyuan;Zhao Bin;Zhou Shengfang;Liu Huan;Sun Minghong;Zhang Feng;Lei Hongtao;Fang Jiliang(Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China;Beijing Key Lab of Plant Resource Research and Development, Beijing Technology and Business University, Beijing 100048, China;Beijing Anzhen Hospital Affiliated to Capital Medical University, Beijing 100037, China;Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China;Medical Experiment Center, China Academy of Chinese Medical Sciences, Beijing 100700, China)

机构地区：[1]中国中医科学院针灸研究所,北京100700 [2]北京工商大学植物资源研究开发北京市重点实验室,北京100048 [3]首都医科大学附属北京安贞医院,北京100037 [4]中国中医科学院广安门医院,北京100053 [5]中国中医科学院医学实验中心,北京100700

出　　处：《世界科学技术-中医药现代化》2019年第5期988-993,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：中华人民共和国科学技术部国家重点基础研究发展计划(973计划)项目(2012CB518503)：经穴效应循经特异性靶器官响应的生物学基础研究;负责人：荣培晶;国家自然科学基金委员会青年项目(81102650)：针灸血清激活不同细胞内钙信号传递通路效应规律及机理研究;负责人：张金铃;北京工商大学北京市植物资源研究开发重点实验室开放课题基金资助课题(PRRD-2016-ZD2)：丹参提取物对寻常痤疮的改善及其预后的影响;负责人：荣培晶(孟宏)

摘　　要：目的:观察埋置电针对慢性心肌缺血模型猪心肌ERK表达的影响,探讨针刺应用于干预大型实验动物猪心肌缺血的方法可行性。方法:实验采用普通级巴马小型猪,分为三组:正常对照组、慢性心肌缺血模型组、针刺组,造模方法为于中华小型猪左冠状动脉左前降支放置Ameroid缩窄环,造模术后4周进行穴位埋针2周并加2次20分钟的电针治疗。术后六周后心脏取材,进行ERK表达及电镜检测。结果:针刺组与模型组比较,ERK1/2的表达均显著增强(**P <0.01);模型组和针刺组比较心肌细胞线粒体损伤评分没有显著性差异;模型组与正常组比较心肌细胞线粒体损伤评分升高(**P <0.01)。结论:手术放置蛋白缩窄环于左前降支制备大型实验动物猪心肌缺血模型方法可行,针刺可能通过调节ERK通路发挥增强机体保护细胞缺血损伤的能力。Objective: To observe the effects of embedded electroacupunctureon ERK expression inchronic myocardial ischemia modelpigs, and to explore thefeasibilityof acupunctureappliedinthe interventioninlargeexperimental pigs withmyocardial ischemia. Methods: OrdinaryBa-Mamini pigsin the experimentweredividedinto3 group: normal control group,chronic myocardial ischemia model group and acupuncture group. In the left anterior descending branch of the left coronary artery of the Chineseminiature pig, the Ameroid protein contraction ring was placed.After 4 weeks of theneedle embedding and 2 times of 20-min electroacupuncture. Six weeks after the modeling, the myocardiac tissue was cut off for the ERK expressionand electron microscopy test. Results:Compared with the chronic myocardial ischemia model group, the expression of ERK1/2 in the acupuncture groupwas increased significantly(**P < 0.01). Compared with theacupuncture group, therewas no sifnificantdifferencein the myocardial cell mitochondrial injury score of the chronic myocardial ischemia modelgroup;compared withthenormal controlgroup, the myocardial cell mitochondrial injury scoreof the chronic myocardial ischemia model group was significantly increased(**P < 0.01). Conclusion: It is feasible to set up the large experimental pig model of myocardial ischemia bythe operation of placingthe protein contraction ringin the left anterior descending branch. Acupuncture can regulate the ERK pathway to enhance the body to protect the cellsfrom ischemic injury.

关 键 词：大型实验动物猪 慢性心肌缺血 埋置电针 ERK蛋白表达 

分 类 号：R259[医药卫生—中西医结合]