Klotho基因多态性与60岁以上老年原发性骨质疏松症的相关性  

Association of polymorphisms of klotho gene with primary osteoporosis in the elderly aging above 60 years old

作　　者：马秋华[1] 王云文[1] 马慧[1] 齐杰 张清潭[1] Ma Qiuhua;Wang Yunwen;Ma Hui;Qi Jie;Zhang Qingtan(Binzhou Medical University Hospital, Binzhou 256600,Shandong Province, China)

机构地区：[1]滨州医学院附属医院

出　　处：《中国组织工程研究》2019年第35期5587-5591,共5页Chinese Journal of Tissue Engineering Research

基　　金：滨州医学院校级课题(BY2015KJ22)，项目负责人：马秋华~~

摘　　要：背景:研究发现,Klotho基因与人类多种疾病如骨质疏松、肿瘤、动脉粥样硬化、血糖异常等均有关。目的:进一步验证klotho基因多态性与老年人(≥60岁)原发性骨质疏松症的相关性。方法:研究方案的实施符合滨州医学院附属医院医院对研究的相关伦理要求。受试者对试验过程完全知情同意。收集于滨州医学院附属医院体检中心、老年医学科就诊的,长期居住鲁西北地区的汉族老年患者609例,其中骨质疏松者303例,骨量正常者306例,利用SNaPshotSNP分型技术对klotho基因G395A、F352V位点进行基因分型。结果与结论:①Klotho基因G395A基因型GG在骨量正常组中分布频率显著高于骨质疏松组(P<0.05);②女性骨质疏松组患者GG基因型分布频率显著低于骨量正常组(P<0.05);③多因素Logistic回归分析表明,总胆红素、年龄及体质量指数为原发性骨质疏松症发病的危险因素;④结果说明,Klotho基因G395A、F352V位点多态性可能不是该地区汉族老年人群骨质疏松症发生的危险因子,总胆红素、年龄及体质量指数可能与骨质疏松症的发生有关。BACKGROUND: Klotho gene has been shown to be related to various diseases, such as osteoporosis, tumor, atherosclerosis and dyslipidemia. OBJECTIVE: To further testify the association of polymorphisms of klotho gene with primary osteoporosis in elderly(≥ 60 years old). METHODS: The study was in accordance with the ethical requirements of Binzhou Medical University Hospital, and the participants signed the informed consents. A total of 609 elderly Han patients were selected from Medical Examination Center and Division of Geriatrics of Binzhou Medical University Hospital. There were 303 osteoporotic patients, and 306 healthy controls. Genotypes of klotho G395A and F352 V polymorphisms were analyzed by multiplex SNaPshot SNP reaction. RESULTS AND CONCLUSION:(1) The distribution frequency of GG genotype of klotho G395A in the control group was higher than that in the osteoporosis group(P < 0.05).(2) The distribution frequency of GG genotype of klotho G395A in the female osteoporosis group was lower than that in the control group(P < 0.05).(3) According to multivariate logistic regression analysis, the total bilirubin, age and body mass index were risk factors of primary osteoporosis.(4) In summary, the klotho loci G395A and F352V polymorphisms may be not the risk factors of osteoporosis in the Han elderly in this area. Total bilirubin, age and body mass index may be related to osteoporosis.

关 键 词：KLOTHO基因 老年人 骨质疏松症 总胆红素 骨密度 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]