穿骨道缝线桥技术与改良Mason-Allen技术修复单侧兔肩袖损伤模型  

Tranosseous suture bridge technique versus modified Mason-Allen technique repairs unilateral rotator cuff tears in rabbits

作　　者：寇景浩 蒲劲松[1] 蒋成[1] 刘涛[1] 雷小雨 Kou Jinghao;Pu Jingsong;Jiang Cheng;Liu Tao;Lei Xiaoyu(Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, Sichuan Province, China;North Sichuan Medical College, Nanchong 637000,Sichuan Province, China)

机构地区：[1]川北医学院附属医院

出　　处：《中国组织工程研究》2019年第35期5605-5610,共6页Chinese Journal of Tissue Engineering Research

基　　金：四川省卫生和计划生育委员会科研基金(17PJ201)，项目负责人：蒲劲松~~

摘　　要：背景:肩袖损伤修复的小动物模型多采用改良Mason-Allen技术,缝线桥技术鲜有报道。目的:为小动物实验探索一种修复肩袖损伤的新方法。方法:实验方案经川北医学院动物实验伦理委员会批准。48只雄性新西兰大白兔,随机分为对照组及实验组,均建立左侧肩袖损伤模型。对照组行改良Mason-Allen技术修复、实验组行穿骨道缝线桥技术修复。造模后4,8,12周分别随机处死,取肱骨近端标本,行大体标本观察、组织学观察及评分。结果与结论:①大体标本观察:对照组2例固定失效;②组织学观察:实验组造模后12周时软骨细胞排列较整齐,胶原纤维排列致密、整齐,趋于正常的柱状结构;对照组造模后12周时腱骨界面以瘢痕愈合为主,软骨细胞较少胶原纤维排列紊乱;③在4,8,12周时实验组腱骨愈合成熟度评分明显优于对照组(P<0.01);④结果说明,穿骨道缝线桥技术较改良Mason-Allen技术腱骨愈合成熟度评分更高,固定失效率更低,提高了肩袖腱骨愈合率,是一种较好的修复肩袖的动物模型。BACKGROUND: The modified Mason-Allen technique is commonly used for repairing rotator cuff tears in small animals, while the suture bridge technique is rarely reported. OBJECTIVE: To explore a new method for repairing rotator cuff injury in small animals. METHODS: The study was approved by the Experimental Animal Ethics Committee of North Sichuan Medical College. Forty-eight male New Zealand white rabbits were used to make rotator cuff injury models and randomly divided into control(repaired by Mason-Allen technique) and experimental(repaired by tranosseous suture bridge technique) groups. Sixteen animals were sacrificed at 4, 8, and 12 weeks, respectively, and the proximal humerus specimens were used for gross observation, histological examination, and modified maturity of tendon-bone healing score. RESULTS AND CONCLUSION:(1) Gross observation: there were two cases of fixation failure in the control group.(2) Histological examination: at 12 weeks after modeling, in the experimental group, the chondrocytes arranged regularly, and fibrocartilage arranged densely and regularly, which similar with columnar structure. The tendon-bone interface in the control group revealed scar healing mainly, and few chondrocytes and fibrocartilage that arranged in disorder were observed.(3) At 4, 8 and 12 weeks, the scores of tendon-bone healing in the experimental group were significantly better than those in the control group(P < 0.01).(4) These results indicate that compared with modified Mason-Allen technique, the transosseous suture bridge technique possesses higher maturity of tendon-bone healing score and lower fixation failure rate, and improves the rotator cuff tendon-bone healing rate, which is a better method to repair rotator cuff injury in animal models.

关 键 词：肩袖损伤 腱骨愈合 穿骨隧道 缝线桥 动物造模 兔 

分 类 号：R446[医药卫生—诊断学] R686.5[医药卫生—临床医学]