膝关节应力负荷减少对小鼠早期骨关节炎软骨和软骨下骨的影响  

Effect of reduction of mechanical loading on subchondral bone and articular cartilage of early osteoarthritis in mice

作　　者：邵毅杰 姜华晔 高超[1] 罗宗平[2] 杨惠林[1] Shao Yijie;Jiang Huaye;Gao Chao;Luo Zongping;Yang Huilin(the First Affiliated Hospital of Soochow University, Suzhou 215000,Jiangsu Province, China;Institute of Orthopedics at Soochow University, Suzhou 215000, Jiangsu Province, China)

机构地区：[1]苏州大学附属第一医院,江苏省苏州市215000 [2]苏州大学骨科研究所,江苏省苏州市215000

出　　处：《中国组织工程研究》2019年第35期5611-5618,共8页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(81320108018,31570943,31270995)，项目负责人：罗宗平~~

摘　　要：背景:骨关节炎目前在全球发病率较高,但其内在发病机制研究仍不透彻。目的:探究膝关节应力负荷减少对早期骨性关节炎小鼠软骨和软骨下骨的影响。方法:经苏州大学附属第一医院实验动物伦理委员会批准,选取48只雄性成年(8周龄)C57BL/6小鼠,随机分为正常组、关节炎模型组、运动神经切断+模型组以及运动神经切断+假手术组,每组12只。骨关节炎造模前1周进行胫神经联合腓总神经横断,造模后4,8周每组分别处死6只小鼠,收集每组小鼠的右侧膝关节标本,分别进行Micro-CT扫描、组织学染色、足印分析及肌肉萎缩检测。结果与结论:①造模后4周及8周,运动神经切断+模型组中骨体积分数值低于关节炎模型组(P<0.05)。运动神经切断+模型组骨体积分数值与运动神经切断+假手术组差异无显著性意义(P>0.05)。造模后8周运动神经切断+模型组骨体积分数值高于造模后4周(P<0.05);②造模后4周及8周,运动神经切断+模型组中的OARSI评分低于关节炎模型组(P<0.05)。造模后8周,运动神经切断+模型组中的OARSI评分高于造模后4周(P<0.05);③相比于关节炎模型组,造模后4周及8周运动神经切断+模型组中后足足宽减少(P<0.05);右胫前肌及右小腿三头肌湿质量减少(P<0.05);④结果表明,膝关节负荷减少后,小鼠软骨下骨骨量明显降低,关节软骨层厚度减少,提示适宜的关节负荷对维持关节软骨和软骨下骨的稳态有着重要的作用。BACKGROUND: Osteoarthritis has a high incidence in the world, but its underlying pathogenesis is still not thoroughly studied. OBJECTIVE: To investigate the effect of reduction of mechanical loading on subchondral bone and articular cartilage of early osteoarthritis in mice. METHODS: The study was approved by the Laboratory Animal Ethics Committee of the First Affiliated Hospital of Soochow University. Forty-eight male adult C57BL/6 mice(8 weeks old) were randomly divided into normal group, osteoarthritis model group, motor nerve transection+osteoarthritis model group, and motor nerve transection+sham group, 12 mice in each group. We simulated the reduction of mechanical loading in knee joint via tibial nerve combined with common peroneal nerve transection, which was performed at 1 week before the osteoarthritis induction. Six mice in each group were sacrificed at 4 and 8 weeks after modeling. The right knee joints of each group were collected for micro-CT scanning, histological staining, footprint analysis and muscle atrophy detection, respectively. RESULTS AND CONCLUSION:(1) The bone volume fraction in the motor nerve transection+osteoarthritis model group was lower than that in the osteoarthritis model group at 4 and 8 weeks after modeling(P < 0.05). There was no significant difference in the bone volume fraction between motor nerve transection+osteoarthritis model and motor nerve transection+sham groups(P > 0.05). The bone volume fraction in the motor nerve transection+osteoarthritis model group was increased significantly at 8 weeks compared with that at 4 weeks after modeling(P < 0.05).(2) The OARSI score in the motor nerve transection+osteoarthritis model group was lower than that in the osteoarthritis model group at 4 and 8 weeks(P < 0.05). OARSI score in the motor nerve transection+osteoarthritis model group at 8 weeks was higher than that at 4 weeks after modeling(P < 0.05).(3) Compared with the osteoarthritis model group, the hindfoot base widths in the motor nerve transection+osteoarthritis model grou

关 键 词：骨关节炎 应力负荷 关节软骨 软骨下骨 MICRO-CT 足印分析 骨体积分数 国家自然科学基金 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]