影响大鼠自体脂肪组织移植后留存体积的变量因素  

Variables affecting the retained volume of adipose tissue after transplantation in rats

作　　者：贾大平 马莉[1] 吴亮亮[1] 赵宇[1] Jia Daping;Ma Li;Wu Liangliang;Zhao Yu(Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei 230022,Anhui Province, China)

机构地区：[1]安徽医科大学第一附属医院整形外科

出　　处：《中国组织工程研究》2019年第35期5652-5657,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81171829)，项目负责人：赵宇~~

摘　　要：背景:自体颗粒脂肪组织移植后高且不稳定的吸收率限制了其临床运用,除早期建立血供的方法外,寻找新的提升存活率的途径尤为重要。目的:从细胞凋亡角度探究机械剪切及抗凋亡药物依达拉奉对自体脂肪组织移植后体积留存的影响。方法:实验方案经安徽医科大学实验动物伦理委员会批准。取42只SD雄性大鼠随机分成A、B、C3组,模拟临床自体脂肪组织移植行大鼠脂肪组织移植。A组为对照组,6只大鼠,移植中给予脂肪组织130次基础剪切次数;B组又细分为B1、B2和B3组,分别给予脂肪组织230,330,及430次机械剪切;C组细分为C1、C2和C3亚组,分别注射给予脂肪0.1%、0.2%及1%浓度依达拉奉1mL处理5min后,再给予脂肪组织130次剪切次数。于脂肪组织移植后即时(术中收获脂肪时留取)及1,4,8周取标本,对标本形态学特征及体积观察记录、苏木精-伊红染色后光镜下观察组织学改变、应用流式细胞仪AnnexinV/PI双染法检测比较各组脂肪细胞凋亡比例。结果与结论:①与对照组比较:B组移植后即时和1周时标本细胞凋亡比例明显增多,移植物吸收速率快;移植后4,8周时苏木精-伊红染色显示脂肪细胞体积大小不一、排列紊乱,移植物留存体积减少(P<0.05);C1和C2组移植后即时和1周时标本的细胞凋亡比例明显降低,移植后4,8周时脂肪细胞形态较规则、体积较一致、可见较多血管增生、移植物留存体积明显增多(P<0.05);②C3组移植后脂肪吸收率与同期对照组比较差异无显著性意义(P>0.05);③由此得出结论,自体脂肪组织移植中,机械剪切会减少移植后脂肪组织的留存体积,一定浓度的抗凋亡药物依达拉奉能增加移植后脂肪组织的留存体积。BACKGROUND: The high and unstable absorption rate after autologous granule fat transplantation limits its clinical application. In addition to the early establishment of blood supply, it is particularly important to find new ways to improve survival rate. OBJECTIVE: To investigate the effect of mechanical shearing and anti-apoptotic drug edaravone on the volume retention after autologous fat transplantation from the perspective of apoptosis. METHODS: The study was approved by the Laboratory Animal Ethics Committee of Anhui Medical University. Forty-two Sprague-Dawley male rats were randomly divided into three groups: A, B, and C. The rats were transplanted with fat by clinical autologous fat transplantation. Group A was the control group(n=6): the adipose tissue was given 130 times of basic shearing during operation;the B and C groups were given different variables of adipose tissue on the basis of group A. Group B was a mechanical shearing group with a total of 18 rats subdivided into subgroups B1, B2 and B3, which were given 230 times, 330 times and 430 mechanical shears, respectively. Group C was the edaravone group(n=18), subdivided into C1, C2 and C3 subgroups, respectively, given 0.1%, 0.2% and 1% concentration of edaravone, and then the adipose tissue was given 130 times of basic shearing. Graft specimens were taken immediately after operation and at 1, 4 and 8 weeks after transplantation, to observe and record the morphological features and volume of the specimens. The histological changes were observed under a light microscope after hematoxylin-eosin staining. The apoptosis ratio of fat cells in each group was detected by flow cytometry Annexin V/PI double staining. RESULTS AND CONCLUSION:(1) Compared with the control group, the group B: the proportion of apoptosis was increased significantly at immediately and 1 week after surgery, and the graft absorption rate was fast;at 4 and 8 weeks after operation, the volume of hematoxylineosin-stained adipocytes was different, the arrangement was disordered, and

关 键 词：脂肪细胞 移植 细胞凋亡 依达拉奉 脂肪组织 移植物吸收 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]