IL-37对巨噬细胞吞噬杀伤结核杆菌能力的影响  

Effects of IL-37 on phagocytosis and killing ability of macrophages for mycobacterium tuberculosis

作　　者：陈思达[1] 卓宋明[1] 李娜[1] 吴雨梅 黄震[1] 季乐财 CHEN Sida;ZHUO Songming;LI Na;WU Yumei;HUANG Zhen;JI Lecai(Department of Respiratory Medicine,Longgang District Center Hospital,Guangdong,Shenzhen 518116,China;Baoan Community Health Service Cener,the Third People’s Hospital of Longgang District,Guangdong,Shenzhen 518115,China)

机构地区：[1]深圳市龙岗中心医院呼吸内科,广东深圳518116 [2]深圳市龙岗区第三人民医院保安社区健康服务中心,广东深圳518115 [3]深圳市慢性病防治中心,广东深圳518020

出　　处：《中国医药科学》2019年第15期23-26,33,共5页China Medicine And Pharmacy

基　　金：广东省深圳市科技计划项目（JCYJ20160428145728055）;广东省深圳市卫生计生系统科研项目（SZXJ2017035）;广东省深圳市龙岗区科技发展资金（20160607092939550,20160603101543588）

摘　　要：目的研究不同IL-37干预条件下巨噬细胞吞噬杀伤结核分支杆菌能力的变化及其机制。方法体外诱导人单核细胞分化为巨噬细胞。分为四组,对照组:加入非识别siRNA作为空染对照;灭活结核分支杆菌(iH37Rv)组:siRNA空染+iH37Rv刺激;iH37Rv+siIL-37组:siIL-37转染抑制IL-37表达+iH37Rv刺激;iH37Rv+IL-37组:加入外源性IL-37+iH37Rv刺激。按照分组转染siRNA,加入iH37Rv刺激通过酶联免疫吸附法(ELISA)检测上清中IL-37水平,实时荧光定量PCR检测IL-37mRNA,Westernblot检测IL-37蛋白表达,检测NO生成量。罗丹明B标记iH37Rv后,流式细胞术检测巨噬细胞吞噬能力。结果经iH37Rv刺激,iH37Rv组与对照组比较,IL-37分泌量升高,差异有统计学意义(P<0.05);经siRNA转染,iH37Rv+siIL-37组与iH37Rv组比较,IL-37mRNA表达下降,差异有统计学意义(P<0.05);Westernblot检测,与iH37Rv组比较,iH37Rv+siIL-37组IL-37蛋白表达量下降,差异有统计学意义(P<0.05);对照组、iH37Rv组、iH37Rv+siIL-37组及iH37Rv+IL-37组NO分泌量比较,差异有统计学意义(P<0.05);iH37Rv+siIL-37组与iH37Rv组比较,NO分泌量升高,差异有统计学意义(P<0.05);iH37Rv+IL-37组与iH37Rv组比较,NO分泌量下降,差异有统计学意义(P<0.05);与iH37Rv组比较,iH37Rv+siIL-37组巨噬细胞吞噬能力升高,iH37Rv+IL-37组巨噬细胞吞噬能力下降。结论IL-37能抑制巨噬细胞吞噬杀伤结核杆菌,其机制可能是诱导巨噬细胞向M2型极化。Objective To study the changes and mechanisms of phagocytosis and killing ability of macrophages for mycobacterium tuberculosis under different IL-37 intervention conditions. Methods Human monocytes were induced to differentiate into macrophages in vitro and they were divided into 4 groups.Control group:non-identified siRNA was added as a blank control.Inactivated mycobacterium tuberculosis (iH37Rv) group:siRNA empty staining + iH37Rv stimulation. IH37Rv+siIL-37 group:siIL-37 transfection inhibited IL-37 expression and iH37Rv stimulation.IH37Rv+IL-37 group:stimulated by exogenous IL-37 and iH37Rv.The siRNA was transfected into different groups and the IL-37 level in the supernatant was detected by enzyme-linked immunosorbent assay (ELISA).IL-37 mRNA was detected by real-time fluorescent quantitative PCR,IL- 37 protein expression was detected by Western blot and NO production.After rhodamine B was labeled with iH37Rv,flow cytometry was used to detect macrophage phagocytosis. Results After iH37Rv stimulation,the secretion of IL-37 was increased in the iH37Rv group compared with the control group,and the difference was statistically significant (P < 0.05).After transfection with siRNA,the expression of IL-37 mRNA in iH37Rv+ siil-37 group decreased compared with that in iH37Rv group and the difference was statistically significant (P < 0.05).Western blot analysis showed that the expression of IL-37 protein in iH37Rv+siIL-37 group was significantly lower than that in iH37Rv group.The difference was statistically significant (P < 0.05).The differences in NO secretions among the control group,iH37Rv group,iH37Rv+siIL-37 group and iH37Rv+IL-37 group were statistically significant (P < 0.05).Compared with iH37Rv group,the amount of NO secretion increased in iH37Rv+siIL-37 group,and the difference was statistically significant (P < 0.05). Compared with the iH37Rv group,the amount of NO secretion decreased in the iH37Rv+IL-37 group,and the difference was statistically significant (P < 0.05).Compared with the iH37Rv group,th

关 键 词：IL-37 巨噬细胞 结核杆菌 吞噬功能 

分 类 号：R392[医药卫生—免疫学]