长链非编码RNA-ROR在乳腺癌诊断中的潜在应用研究  

Potential application of long-chain non-coding RNA-ROR in breast cancer diagnosis

作　　者：曹鸣菲 雷德财 宋晓玉[2] 吴立春[2] CAO Mingfei;LEI Decai;SONG Xiaoyu;WU Lichun(Department of Clinical Laboratory,Chuankong People′s Hospital,Jianyang,Sichuan 641400,China;Department of Clinical Laboratory,Sichuan Cancer Hospital,Chengdu,Sichuan 610041,China)

机构地区：[1]简阳市川空人民医院检验科,四川简阳641400 [2]四川省肿瘤医院检验科,四川成都610041

出　　处：《国际检验医学杂志》2019年第17期2077-2080,2084,共5页International Journal of Laboratory Medicine

基　　金：四川省科学技术厅重点研发项目(2018SZ0039)

摘　　要：目的 探讨长链非编码RNA-ROR(lincRNA-ROR)在乳腺癌早期诊断中的潜在价值。方法 采用q-PCR检测乳腺癌组织和血清中lincRNA-ROR的表达水平;使用电化学发光法检测样本中癌抗原153(CA153)和癌胚抗原(CAE)的水平。通过建立多元Logistc回归模型绘制受试者工作特征曲线(ROC曲线)分析血清中lincRNA-ROR对早期乳腺癌的潜在诊断价值。结果 LincRNA-ROR在乳腺癌患者组织和外周血清中高表达,对血清中高表达的lincRNA-ROR、CA153及CEA绘制ROC曲线,发现lincRNA-ROR曲线下面积(AUC)为0.76,对乳腺癌诊断价值优于CA153(AUC=0.66)和CEA(AUC=0.52),差异有统计学意义(P<0.0001),且lincRNA-ROR、CA153和CEA联合检测诊断价值(AUC=0.85)高于lincRNA-ROR单独检测。结论 LincRNA-ROR在乳腺癌患者组织和血清中表达上调,并具有良好的灵敏度和特异度,是乳腺癌诊治及其预后监控的潜在生物标志物。Objective To explore the potential value of long-chain non-coding RNA-ROR (lincRNA-ROR) in early diagnosis of breast cancer. Methods The expression of lincRNA-ROR in breast cancer tissues and serum was detected by q-PCR,and the levels of CA153 and CAE in breast cancer samples were detected by electrochemiluminescence.The potential diagnostic value of lincRNA-ROR in serum for early breast cancer was analyzed by establishing multiple logistc regression model and drawing ROC curve. Results LincRNA-ROR was highly expressed in tissues and peripheral serum of breast cancer patients.The ROC curves of lincRNA-ROR,CA153 and CEA were drawn.It was found that the area under the lincRNA-ROR curve (AUC) was 0.76 ,which was superior to CA153 (AUC=0.66) and CEA (AUC=0.52),the difference was statistically significant ( P <0.000 1),and the combined diagnostic value of lincRNA-ROR,CA153 and CEA (AUC= 0.85 ) was higher than that of lincRNA-ROR alone. Conclusion LincRNA-ROR is up-regulated in tissues and serum of breast cancer patients,and has good sensitivity and specificity.It is a potential biomarker for diagnosis,treatment and prognosis monitoring of breast cancer.

关 键 词：长链非编码RNA-ROR 乳腺癌 癌胚抗原 癌抗原153 

分 类 号：R737.9[医药卫生—肿瘤] R446.9[医药卫生—临床医学]