PRA法检测hsp65和groES在非结核分枝杆菌肺病诊断中的价值  

Value of detection of hsp65 and groES by PRA in the diagnosis of non-tuberculosis Mycobacterium pulmonary disease

作　　者：章洁[1] 郭秋萍 ZHANG Jie;GUO Qiuping(Department of Clinical Laboratory,Guangyuan Third People′s Hospital,Guangyuan,Sichuan 628001,China)

机构地区：[1]广元市第三人民医院检验科

出　　处：《国际检验医学杂志》2019年第17期2112-2115,2119,共5页International Journal of Laboratory Medicine

摘　　要：目的 探讨PCR-限制性片段长度多态性分析(PRA)法检测hsp65和groES在非结核分枝杆菌肺病诊断中的价值。方法 选取非结核分枝杆菌肺病患者30例为肺病组,结核病患者30例为结核组,非结核分枝杆菌病感染患者30例为感染组,同时选取体检健康者30例为健康对照组。使用PRA法检测4组受试者痰液中hsp65和groES的相对分子质量。采用受试者工作特征曲线(ROC曲线)下面积分析hsp65和groES联合检测在非结核分枝杆菌肺病中的诊断意义。结果 肺病组患者痰液中hsp65相对分子质量和groES相对分子质量明显高于结核组、感染组及健康对照组(P<0.05)。肺病组与结核组的hsp65和groES灵敏度分别为93%和77%,特异度分别为80%和90%;肺病组与感染组的hsp65和groES的灵敏度分别为77%和63%,特异度分别为63%和96%;肺病组与健康对照组的hsp65和groES的灵敏度分别为93%和76%,特异度分别为83%和87%。hsp65和groES的临界值分别为2.27和1.96。结论 PRA法检测hsp65和groES在非结核分枝杆菌肺病诊断中具有较好的效果。Objective To explore the value of detection of hsp65 and groES by PCR-restriction fragment length polymorphism (PRA) in the diagnosis of non-tuberculosis Mycobacterium pulmonary disease. Methods Thirty patients with non-tuberculosis Mycobacterium pulmonary disease were selected as the pulmonary disease group,30 patients with tuberculosis as the tuberculosis group,and 30 patients with non-tuberculosis Mycobacterium infection as the infection group,at the same time,30 healthy persons were selected as healthy control group.The relative molecular mass of hsp65 and groES in sputum of four groups were measured by PRA.The diagnostic significance of combined detection of hsp65 and groES in non-tuberculosis Mycobacterium pulmonary disease was analyzed under ROC curve. Results The relative molecular weight of hsp65 and groES in sputum of patients with pulmonary disease were significantly higher than those of tuberculosis,infection and health control groups ( P <0.05).The sensitivity and specificity of hsp65 and groES in pulmonary disease group and tuberculosis group were 93% and 77%,80% and 90%,respectively.The sensitivity and specificity of hsp65 and groES in pulmonary disease group and infection group were 77% and 63%,63% and 96%,respectively.The sensitivity and specificity of hsp65 and groES in pulmonary disease group and healthy control group were 93% and 76%,83% and 87% respectively.The critical values of hsp65 and groES were 2.27 and 1.96,respectively. Conclusion Detection of hsp65 and groES by PRA has a good effect in the diagnosis of non-tuberculosis Mycobacterium pulmonary disease.

关 键 词：PCR-限制性片段长度多态性分析 HSP65 GROES 非结核分枝杆菌病 

分 类 号：R563[医药卫生—呼吸系统] R446.5[医药卫生—内科学]