乙醇/水中麦秆盐酸预处理及同步糖化发酵研究  

Study of hydrochloric acid pretreatment in ethanol/water and simultaneous saccharification and fermentation of wheat straw

作　　者：曹拢财 叶艳艳 王佳琪 胡银平 付艺博 祝勇[1] 崔茂金[1] CAO Longcai;YE Yanyan;WANG Jiaqi;HU Yinping;FU Yibo;ZHU Yong;CUI Maojin(School of Chemistry and Chemical Engineering,Henan Institute of Science and Technology,Xinxiang 453003,China)

机构地区：[1]河南科技学院化学化工学院

出　　处：《河南科技学院学报（自然科学版）》2019年第4期36-40,共5页Journal of Henan Institute of Science and Technology(Natural Science Edition)

基　　金：河南科技学院大学生创新训练项目(2018CX64);河南科技学院博士科研启动基金(210010611001)

摘　　要：为了提高麦秆转化为乙醇的效率,通过正交试验对麦秆在乙醇/水中的盐酸预处理条件和乙醇/水中盐酸预处理的麦秆同步糖化发酵条件进行了优化.结果表明:乙醇/水中麦秆盐酸预处理影响的主次顺序为盐酸质量分数、时间、温度、乙醇体积分数和底物质量浓度.乙醇/水中麦秆的最佳盐酸预处理条件为温度120℃、盐酸质量分数8%、时间60min、底物质量浓度2.5g/L和乙醇体积分数40%,此时总还原糖浓度为469.18mg/g.乙醇/水中盐酸对麦秆的预处理效果比水中的要好.乙醇/水中盐酸预处理的麦秆同步糖化发酵影响的主次顺序为酵母接种量、酶浓度、时间和温度.乙醇/水中盐酸预处理的麦秆最佳同步糖化发酵条件为温度33℃、酵母接种量0.3%、酶质量浓度0.8g/L和发酵时间4d,此时乙醇的质量浓度为22.38g/L.乙醇/水中盐酸预处理的麦秆同步糖化发酵制乙醇的效果比水中的要好.In order to increase the efficiency of converting wheat straw into ethanol,the conditions of hydrochloric acid(HCl) pretreatment of wheat straw in ethanol/water and simultaneous saccharification and fermentation(SSF) of wheat straw pretreated by HCl in ethanol/water were optimized by orthogonal experiment.The results showed that the primary and secondary that effected HCl pretreatment of wheat straw in ethanol/water were HCl concentration,time,temperature,ethanol volume percent and substrate mass concentration.The optimal HCl pretreatment conditions of wheat straw in ethanol/water were temperature of 120 ℃,HCl concentration of 8%,time of 60 min,substrate mass concentration of 2.5 g/L and ethanol volume percent of 40%.The total reducing sugar concentration was 469.18 mg/g at the optimal pretreatment conditions.The effect of HCl pretreatment of wheat straw in ethanol/water solvent was better than that in water.The primary and secondary that effected the SSF of wheat straw pretreated by HCl in ethanol/water was yeast inoculation,enzyme concentration,time and temperature.The optimal SSF conditions of wheat straw pretreated by HCl in ethanol/water were temperature of 33 ℃,yeast inoculation of 0.3%,enzyme concentration of 0.8 g/L and fermentation time of 4 d.The ethanol concentration was 22.38 g/L at the optimal SSF conditions.The effect of SSF of wheat straw pretreated by HCl in ethanol/water solvent to ethanol is better than that of water.

关 键 词：麦秆 有机溶剂 酸预处理 同步糖化发酵 正交试验 

分 类 号：X712[环境科学与工程—环境工程] S216.2[农业科学—农业机械化工程]