富硒长双歧杆菌水溶性蛋白保护脂多糖诱导的大鼠肠上皮细胞IEC6损伤  

The protective effect of water-soluble proteins from selenium-enriched Bifidobacterium longum on lipopolysaccharide-induced injury in rat IEC6 cells

作　　者：徐莉莎 朱家桢 冯黎黎 吴旭东 XU Li-sha;ZHU Jia-zhen;Feng Li-li;WU Xu-dong(State Key Lab of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023,China)

机构地区：[1]南京大学生命科学学院医药生物技术国家重点实验室

出　　处：《中国药理学通报》2019年第9期1274-1278,共5页Chinese Pharmacological Bulletin

基　　金：江苏省自然科学基金资助项目(No BK20161399)

摘　　要：目的本研究用脂多糖诱导大鼠肠上皮细胞IEC6损伤模型,考察富硒长双歧杆菌制剂对IEC6的保护作用。方法实验分5组,分别为阴性对照组(磷酸平衡盐溶液处理)、模型组(脂多糖处理)、富硒长双歧杆菌水溶性蛋白低、中、高3个浓度组(脂多糖处理的同时分别加入10、30、100 mg·L^-1的富硒长双歧杆菌水溶性蛋白)。脂多糖处理24 h后,检测IEC6细胞活力、细胞凋亡、线粒体膜电位、细胞紧密连接蛋白1(zonula occludens 1,ZO-1)和闭合素(Occludin)的表达。结果脂多糖可导致大鼠肠上皮细胞IEC6损伤,表现为细胞活力下降,凋亡增加,线粒体膜电位倒塌,细胞紧密连接蛋白表达减少。富硒长双歧杆菌水溶性蛋白能够抑制脂多糖诱导的IEC6细胞损伤,减少细胞凋亡,增强细胞间紧密连接。结论富硒长双歧杆菌水溶性蛋白能够保护脂多糖诱导的IEC6细胞损伤。Aim To investigate the protective effect of selenium-enriched Bifidobacterium longum preparation on rat IEC6 cells via the application of lipopolysaccharide (LPS)-induced IEC6 cell injury model. Methods The experiment was divided into five groups: negative control group treated with phosphoric acid equilibrium salt solution, model group treated with LPS, and low, medium, high concentration group in which IEC6 cells were treated with LPS plus 10, 30, 100 mg·L -1 water-soluble proteins from selenium-enriched Bifidobacterium longum respectively. After treatment with LPS for 24 hours, IEC6 cell viability, apoptosis, mitochondria membrane potential, and the expression of zonula occludens 1 (ZO-1) and Occludin were detected. Results LPS induced rat intestinal epithelial cell damage, such as the decrease of cell viability, the increase of cell apoptosis, the collapse of mitochondria membrane potential, and the decrease of cell tight junction protein expression. Water-soluble proteins from selenium-enriched Bifidobacterium longum inhibited LPS-induced IEC6 cell damage, decreased cell apoptosis and increased cell tight junction between cells. Conclusion Water-soluble proteins from selenium-enriched Bifidobacterium longum have protective effect on LPS-induced rat intestinal epithelial cell injury.

关 键 词：富硒长双歧杆菌水溶性蛋白 脂多糖 大鼠肠上皮细胞 细胞紧密连接 细胞凋亡 线粒体膜电位 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学] R378[医药卫生—基础医学]