外源Ca对旱柳Cd毒害的缓解作用  

Effects of exogenous Ca on the toxicity of Cd in Salix matsudana Koidz

作　　者：李崇豪 薛文秀 尚晓硕 邹金华[1,2] 蒋志勤[3] LI Chonghao;XUE Wenxiu;SHANG Xiaoshuo;ZOU Jinhua;JIANG Zhiqin(College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University, Tianjin 300387, China;College of Chemistry,Tianjin Normal University,Tianjin 300387,China)

机构地区：[1]天津师范大学生命科学学院,天津300387 [2]天津师范大学天津市动植物抗性重点实验室,天津300387 [3]天津师范大学化学学院,天津300387

出　　处：《天津师范大学学报（自然科学版）》2019年第4期58-63,共6页Journal of Tianjin Normal University：Natural Science Edition

基　　金：天津市自然科学基金资助项目(17JCYBJC22500)

摘　　要：为了探究Cd胁迫对旱柳的毒害以及外源Ca的缓解作用,以一年生旱柳枝条为水培材料,设置对照组、Ca处理组(Ca^2+浓度为5 mmol/L)、Cd处理组(Cd^2+浓度为50μmol/L)和Ca^+Cd处理组(Ca^2+和Cd^2+浓度分别为5μmol/L和50 mmol/L)4个处理组,研究Cd胁迫环境中的旱柳在添加了外源Ca后光合色素含量、光合生理、保护酶系统和丙二醛含量等的变化.结果表明:①Cd胁迫作用下,旱柳叶片的光合色素含量明显减少,在添加外源Ca后光合色素含量显著增加;②Cd处理会降低旱柳光合作用参数进而影响旱柳的光合作用进程,而Ca^+Cd处理组中各项光合作用参数有明显恢复;③Cd处理会诱导保护酶活性的升高并增加MDA含量,外源Ca的添加则会降低保护酶活性和MDA的含量.因此认为,外源Ca的添加可以有效缓解Cd胁迫对旱柳的毒害作用.To explore the toxicity of Cd stress on Salix matsudana Koidz and the alleviating effect of exogenous Ca, the one-year old shoots of S. matsudana were adopted as experimental materials, and four treatment groups were set, including control, Ca group([Ca^2+] is 5 mmol/L), Cd group([Cd^2+] is 50 μmol/L), Ca^+ Cd group([Ca^2+] is 5 mmol/L and [Cd^2+] is 50 μmol/L). After the addition of exogenous Ca, the changes of photosynthetic pigments content, photosynthetic physiology, protective enzyme system and malondialdehyde(MDA) content of S. matsudana under Cd stress were studied. The results showed that:①The contents of photosynthetic pigments of S. matsudana were reduced by Cd treatment and increased significantly after the addition of exogenous Ca;②Cd treatment reduced the photosynthetic parameters of S. matsudana and then affected the photosynthesis, and the photosynthesis parameters of Ca^+ Cd group were significantly recovered;③Cd stress can increase the protective enzyme activity and MDA content of S. matsudana, while the exogenous Ca can decrease the protective enzyme activity and MDA content. All these results indicated that exogenous Ca can alleviate the toxic effect of Cd stress on S. matsudana.

关 键 词：旱柳 CD CA 毒害 缓解作用 

分 类 号：Q945[生物学—植物学]