In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria  

作　　者：Jessica de Cassia Tomasi Juliana Degenhardt-Goldbach Renata Lucia Grunennvaldt Germana Davila dos Santos Vania Aparecida Vicente Luziane Franciscon Cleusa Bona Marguerite Quoirin 

机构地区：[1]Department of Crop Production and Protection,Federal University of Parana,1540 Rua dos Funciona′rios,Curitiba,PR 80035-060,Brazil [2]Brazilian Agricultural Research Corporation(EMBRAPA)Forestry,Colombo,PR,Brazil [3]Department of Basic Pathology,Federal University of Parana,Curitiba,Brazil [4]Department of Botany,Federal University of Parana,Curitiba,Brazil

出　　处：《Journal of Forestry Research》2019年第5期1765-1777,共13页林业研究（英文版）

基　　金：Brazilian Agricultural Research Corporation(EMBRAPA)Forestry

摘　　要：Yerba mate(Ilex paraguariensis A.St.-Hil.)is a species of great economic,social and environmental importance for the southern regions of Brazil,Uruguay and Argentina.Currently the most diverse products are obtained from mate leaves,including mate tea.The objective of this study was to establish shoot meristem cultures(meristematic dome and a few primordia)of elite clones and identify the endophytic bacteria present in the explants.We tested the effect of clones(F1,F2,A03 and A07),culture media(MS,1/2MS,1/4MS and WPM),cytokinins(kinetin,BA and 2iP),activated charcoal(1,2 and 3 g L^-1),and disinfecting agent(sodium hypochlorite and mercuric chloride)on in vitro establishment.F1 and F2 clones were the most responsive for shoot meristem in vitro culture.WPM medium supplemented with 8.8 lM 2iP,0.2 lM NAA and 3 g L^-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone.No phytotoxic effect of the disinfectant was observed and some meristems sprouted.The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larrymoorei.To conclude,we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response.Yerba mate（Ilex paraguariensis A.St.-Hil.）is a species of great economic,social and environmental importance for the southern regions of Brazil,Uruguay and Argentina.Currently the most diverse products are obtained from mate leaves,including mate tea.The objective of this study was to establish shoot meristem cultures（meristematic dome and a few primordia）of elite clones and identify the endophytic bacteria present in the explants.We tested the effect of clones（F1,F2,A03 and A07）,culture media（MS,1/2 MS,1/4 MS and WPM）,cytokinins（kinetin,BA and 2 iP）,activated charcoal(1,2 and 3 g L-1),and disinfecting agent（sodium hypochlorite and mercuric chloride）on in vitro establishment.F1 and F2 clones were the most responsive for shoot meristem in vitro culture.WPM medium supplemented with 8.8μM 2 iP,0.2 μM NAA and 3 g L-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone.No phytotoxic effect of the disinfectant was observed and some meristems sprouted.The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larry,moorei.To conclude,we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response.

关 键 词：Yerba MATE Tissue culture 2iP ACTIVATED charcoal ENDOPHYTIC BACTERIA AGROBACTERIUM larrymoorei Oxidation 

分 类 号：S[农业科学]