复合微生物菌肥主要功能菌发酵培养优化研究  

Culture Medium and Fermentation Process Optimization of Function Bacteria for Compound Microbial Fertilizer

作　　者：金开铭 田彬 盛玉珍[3] 熊咏梅 徐磊 张玲 王丽[3] JIN Kai-ming;TIAN Bin;SHENG Yu-zhen;XIONG Yong-mei;XU Lei;ZHANG Ling;WANG Li(Forestry Bureau of Xide Country, Xide 616750, China;Panzhihua Branch of the Yangtze River Forestation Bureau, Panzhihua 617000, China;Sichuan Academy of Forestry, Chengdu 610081, China)

机构地区：[1]喜德县林业局,四川喜德616750 [2]长江造林局攀枝花分局,四川攀枝花617000 [3]四川省林业科学研究院,四川成都610081

出　　处：《四川林业科技》2019年第4期17-22,27,共7页Journal of Sichuan Forestry Science and Technology

基　　金：四川省财政专项“基于微生物修复的退化紫色土土壤质量及生态风险评价”(2019CZZX35)

摘　　要：通过优化复合微生物菌肥主要功能菌的发酵培养基配方及其发酵条件,提高发酵液中功能菌的活菌含量,以期为大规模生产复合微生物菌肥奠定基础。利用单因素实验结合正交的方法,以菌体生长量(OD600)为测定指标,对复合微生物菌肥主要功能菌中的固氮菌、溶磷菌和解钾菌的发酵培养基进行优化。结论:①固氮菌培养基的最优组合为麦芽糖7.5g、蛋白胨15g、NaH2PO4·H2O0.3g、K2HPO40.5g、FeCl30.2g、MgSO4·7H20.5g,最佳pH值6.8,最佳接种量为100mL,最佳转速为180r·min-1最佳发酵温度为30℃;②溶磷菌培养基的最优组合为麦芽糖7.5g、蛋白胨15g、NaH2PO4·H2O0.3g、K2HPO40.5g、FeCl30.5g、MgSO4·7H20.5g,最佳pH值6.4,最佳接种量为300mL,最佳转速为180r·min^-1最佳发酵温度为30℃;③解钾菌培养基的最优组合为甘露醇7.5g、牛肉膏15g、NaH2PO4·H2O0.3g、K2HPO40.5g、FeCl30.5g、MgSO4·7H20.5g,最佳pH值7.6,最佳接种量为100mL,最佳转速为180r·min^-1最佳发酵温度为34℃。In this paper culture medium and fermentation process of function bacteria was optimized to improve the living bacterium content for compound microbial fertilizers, and in the purpose of contribution to the basis for the mass production of compound microbial fertilizer. The experiments with one factor and orthogonal design were conducted to optimize culture medium and fermentation process of nitrogen-fixing bacteria, phosphate-solubilizing bacteria and potassium-solubilizing bacteria for compound microbial fertilizers. The results showed that (1) the optimal medium for nitrogen-fixing bacteria was 7.5 g maltose, 15 g peptone, 0.03 g NaH 2PO 4·H 2O, 0.5 g K 2HPO 4, 0.2 g MnSO 4·2H 2O, 0.2 g FeCl 3 and 0.5 g MgSO 4 ·7H 2O, and the optimal culture condition included initial medium pH of 6.4 with inoculum size of 100 mL, rotation speed of 180 r·min^-1 , and the optimal fermentation condition was 30℃.(2) the optimal medium for phosphate-solubilizing bacteria was 7.5 g maltose, 15 g peptone, 0.03 g NaH 2PO 4·H 2O, 0.5 g K 2HPO 4, 0.2 g MnSO 4·2H 2O, 0.5 g FeCl 3, 0.5 g MgSO 4·7H 2O,and the optimal culture condition included initial medium pH of 6.8 with inoculum size of 300 mL, rotation speed of 180 r·min^-1 , and the optimal fermentation condition was 30℃.(3) the optimal medium for potassium-solubilizing bacteria was 7.5 g mannitol, 15 g beef extract, 0.03 g NaH 2PO 4·H 2O, 0.5 g K 2HPO 4, 0.2 g MnSO 4·2H 2O, 0.5 g FeCl 3, 0.5 g MgSO 4·7H 2O,and the optimal culture condition included initial medium pH of 7.6 with inoculum size of 100 mL, rotation speed of 180 r·min^-1, and the optimal fermentation condition was 34℃.

关 键 词：固氮菌 溶磷菌 解钾菌 培养基 优化 

分 类 号：S714.3[农业科学—林学]